CAPZA1 Polyclonal antibody

CAPZA1 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 55145-1-AP

Synonyms

CAPPA1, CAPZ, CapZ alpha 1, CAPZA1, CAZ1



Tested Applications

Positive WB detected inhuman heart tissue, mouse brain tissue, mouse heart tissue
Positive IHC detected inhuman colon cancer tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

55145-1-AP targets CAPZA1 in WB, IHC,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name capping protein (actin filament) muscle Z-line, alpha 1
Calculated molecular weight 33 kDa
Observed molecular weight 34-40 kDa
GenBank accession numberNM_006135
Gene symbol CAPZA1
Gene ID (NCBI) 829
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

CAPZA1 belongs to the F-actin-capping protein alpha subunit family. F-actin-capping proteins bind in a Ca2+-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends. Unlike other capping proteins (such as gelsolin and severin), these proteins do not sever actin filaments. The potential for CAPZ is a therapeutic target in the management of heart failure. Future research is required to determine the mechanisms by which changes in CAPZ impact myocardial function and intracellular signalling, and to develop feasible strategies that can manipulate CAPZ in the intact heart. (PMID: 15614336 ) This antibody is specific to CAPZA1.

Protocols

Product Specific Protocols
WB protocol for CAPZA1 antibody 55145-1-APDownload protocol
IHC protocol for CAPZA1 antibody 55145-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols