CAPZA2 Polyclonal antibody

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  WB analysis of mouse brain using 15948-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15948-1-AP (CAPZA2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 15948-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 15948-1-AP (CAPZA2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 15948-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15948-1-AP (CAPZA2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 15948-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15948-1-AP (CAPZA2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 15948-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15948-1-AP (CAPZA2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 15948-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 15948-1-AP (CAPZA2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 15948-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15948-1-AP (CAPZA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse ovary using 15948-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 15948-1-AP (CAPZA2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 15948-1-AP

  IP result of anti-CAPZA2 (IP:15948-1-AP, 4ug; Detection:15948-1-AP 1:800) with mouse heart tissue lysate 4000ug.

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  IHC staining of human colon cancer using 15948-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 15948-1-AP (CAPZA2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human colon cancer using 15948-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 15948-1-AP (CAPZA2 antibody) at dilution of 1:50 (under 10x lens).

• Featured Product
• KD/KO Validated

CAPZA2 Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 15948-1-AP

Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Synonyms

CAPPA2, CAPZ, CapZ alpha 2, CAPZA2

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Tested Applications

• Positive WB detected in: mouse brain tissue, HepG2 cells, MCF-7 cells, mouse heart tissue, mouse liver tissue, mouse ovary tissue, mouse skeletal muscle tissue
• Positive IP detected in: mouse heart tissue
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below

Product Information

15948-1-AP targets CAPZA2 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag8544

  Product name: Recombinant human CAPZA2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-286 aa of BC005338

  Sequence: MADLEEQLSDEEKVRIAAKFIIHAPPGEFNEVFNDVRLLLNNDNLLREGAAHAFAQYNLDQFTPVKIEGYEDQVLITEHGDLGNGKFLDPKNRICFKFDHLRKEATDPRPCEVENAVESWRTSVETALRAYVKEHYPNGVCTVYGKKIDGQQTIIACIESHQFQAKNFWNGRWRSEWKFTITPSTTQVVGILKIQVHYYEDGNVQLVSHKDIQDSLTVSNEVQTAKEFIKIVEAAENEYQTAISENYQTMSDTTFKALRRQLPVTRTKIDWNKILSYKIGKEMQNA

• Full Name: capping protein (actin filament) muscle Z-line, alpha 2
• Calculated Molecular Weight: 286 aa, 33 kDa
• Observed Molecular Weight: 33-40 kDa
• GenBank Accession Number: BC005338
• Gene Symbol: CAPZA2
• Gene ID (NCBI): 830
• RRID: AB_2070148
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P47755
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Elife; HIPK4 is essential for murine spermiogenesis.; Authors - J Aaron Crapster
mouse	WB	Oncotarget; Genes co-amplified with ERBB2 or MET as novel potential cancer-promoting genes in gastric cancer.; Authors - Mi Jeong Kwon ; KD Validated
human	WB	Biochem J; Cytoskeleton regulators CAPZA2 and INF2 associate with CFTR to control its plasma membrane levels under EPAC1 activation.; Authors - João D Santos
mouse	WB	Eur J Cell Biol; Nuclear receptor Nur77 regulates immunomechanics of macrophages; Authors - Sanne C Lith