CAPZA2 Polyclonal antibody

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• CAPZA2 Polyclonal antibody (15948-1-AP)

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• KD/KO Validated

CAPZA2 Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 15948-1-AP Copied!

Publications (7)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Synonyms

CAPPA2, CAPZ, CapZ alpha 2, CAPZA2

• 

  WB analysis of mouse brain using 15948-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15948-1-AP (CAPZA2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse heart using 15948-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 15948-1-AP (CAPZA2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse skeletal muscle using 15948-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15948-1-AP (CAPZA2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HepG2 using 15948-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15948-1-AP (CAPZA2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of MCF-7 using 15948-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 15948-1-AP (CAPZA2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse liver using 15948-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15948-1-AP (CAPZA2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse ovary using 15948-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 15948-1-AP (CAPZA2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse skeletal muscle using 15948-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15948-1-AP (CAPZA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of mouse heart using 15948-1-AP

  IP result of anti-CAPZA2 (IP:15948-1-AP, 4ug; Detection:15948-1-AP 1:800) with mouse heart tissue lysate 4000ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human colon cancer using 15948-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 15948-1-AP (CAPZA2 antibody) at dilution of 1:50 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human colon cancer using 15948-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 15948-1-AP (CAPZA2 antibody) at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: mouse brain tissue, HepG2 cells, MCF-7 cells, mouse heart tissue, mouse liver tissue, mouse ovary tissue, mouse skeletal muscle tissue
• Positive IP detected in: mouse heart tissue
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15948-1-AP targets CAPZA2 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag8544

  Product name: Recombinant human CAPZA2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-286 aa of BC005338

  Sequence: MADLEEQLSDEEKVRIAAKFIIHAPPGEFNEVFNDVRLLLNNDNLLREGAAHAFAQYNLDQFTPVKIEGYEDQVLITEHGDLGNGKFLDPKNRICFKFDHLRKEATDPRPCEVENAVESWRTSVETALRAYVKEHYPNGVCTVYGKKIDGQQTIIACIESHQFQAKNFWNGRWRSEWKFTITPSTTQVVGILKIQVHYYEDGNVQLVSHKDIQDSLTVSNEVQTAKEFIKIVEAAENEYQTAISENYQTMSDTTFKALRRQLPVTRTKIDWNKILSYKIGKEMQNA

• Full Name: capping protein (actin filament) muscle Z-line, alpha 2
• Calculated Molecular Weight: 286 aa, 33 kDa
• Observed Molecular Weight: 33-40 kDa
• GenBank Accession Number: BC005338
• Gene Symbol: CAPZA2
• Gene ID (NCBI): 830
• RRID: AB_2070148
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P47755
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The CAPZA2 Polyclonal antibody (Cat# 15948-1-AP) has a total of 7 citations appearing in Acta Neuropathologica Communications, Oncotarget, European Journal of Cell Biology, Biochemical Journal, eLife, and bioRxiv. Associated research fields include neurodegeneration (ALS, Alzheimer's disease), gastric cancer, macrophage immunology, CFTR plasma membrane regulation, murine spermiogenesis, and microglial endo-lysosome function.

Automatically generated from the 7 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Acta Neuropathol Commun; Identification and validation of a tear fluid-derived protein biomarker signature in patients with amyotrophic lateral sclerosis.; Authors - Lena-Sophie Scholl
mouse	WB	Oncotarget; Genes co-amplified with ERBB2 or MET as novel potential cancer-promoting genes in gastric cancer.; Authors - Mi Jeong Kwon ; KD Validated
mouse	WB	Eur J Cell Biol; Nuclear receptor Nur77 regulates immunomechanics of macrophages; Authors - Sanne C Lith
human	WB	Biochem J; Cytoskeleton regulators CAPZA2 and INF2 associate with CFTR to control its plasma membrane levels under EPAC1 activation.; Authors - João D Santos
mouse	WB	Elife; HIPK4 is essential for murine spermiogenesis.; Authors - J Aaron Crapster
human	WB	bioRxiv; INPP5D/SHIP1 is a dual regulator of endo-lysosome function and selective phagocytosis in human microglia.; Authors - Gizem Terzioglu