CAPZA3 Polyclonal antibody

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• CAPZA3 Polyclonal antibody (17103-1-AP)

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  WB analysis using 17103-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17103-1-AP (CAPZA3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

CAPZA3 Polyclonal Antibody for WB, ELISA

Cat No. 17103-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

CAPAA3, CAPPA3, CapZ alpha-3, CP-alpha-3, F-actin-capping protein subunit alpha-3

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Tested Applications

• Positive WB detected in: mouse testis tissue, rat testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

17103-1-AP targets CAPZA3 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag10774

  Product name: Recombinant human CAPZA3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-299 aa of BC016745

  Sequence: MTLSVLSRKDKERVIRRLLLQAPPGEFVNAFDDLCLLIRDEKLMHHQGECAGHQHCQKYSVPLCIDGNPVLLSHHNVMGDYRFFDHQSKLSFKYYLLQNQLKDIQSHGIIQNEAEYLRVVLLCALKLYVNDHYPKGNCNMLRKTVKSKEYLIACIEDHNYETGECWNGLWKSKWIFQVNPFLTQVTGRIFVQAHFFRCVNLHIEISKDLKESLEIVNQAQLALSFARLVEEQENKFQAAVLEELQELSNEALRKILRRDLPVTRTLIDWHRILSDLNLVMYPKLGYVIYSRSVLCNWII

• Full Name: capping protein (actin filament) muscle Z-line, alpha 3
• Calculated Molecular Weight: 299 aa, 35 kDa
• Observed Molecular Weight: 30-35 kDa
• GenBank Accession Number: BC016745
• Gene Symbol: CAPZA3
• Gene ID (NCBI): 93661
• RRID: AB_3741905
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96KX2
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CAPZA3 encodes an actin capping protein, one of the F-actin capping protein alpha subunit family. In vertebrates, the α subunit is encoded by three CAPZA genes, CAPZA1, CAPZA2 and CAPZA3, which encode α1, α2 and α3 subunits, respectively(PMID: 32338762). CAPZA3 is expressed exclusively in the testis and sperm(PMID: 12029070).