CARD11 Polyclonal antibody

CARD11 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 28483-1-AP

Synonyms

BIMP3, CARD11, Carma 1, CARMA1



Tested Applications

Positive WB detected inK-562 cells, Raji cells
Positive IHC detected inhuman colon cancer tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

28483-1-AP targets CARD11 in WB, IHC,ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CARD11 fusion protein Ag29355
Full Name caspase recruitment domain family, member 11
Calculated molecular weight 1154 aa, 133 kDa
Observed molecular weight 140-145 kDa
GenBank accession numberBC111719
Gene symbol CARD11
Gene ID (NCBI) 84433
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CARD11 also named as ,BIMP3 or CARMA1, is a 1154 amino acid protein, which contains one PDZ domain, one guanylate kinase-like domain, and one CARD domain. CARD11 localizes in the cytoplasm and co-localized with DPP4 in membrane raft. CARD11 is detected in adult peripheral blood leukocytes, thymus, spleen and liver. CARD11 also is found in CARD11 promyelocytic leukemia HL-60 cells, chronic myelogenous leukemia K-562 cells, Burkitt's lymphoma Raji cells and colorectal adenocarcinoma SW480 cells. CARD11 is involved in the co-stimulatory signal essential for T-cell receptor mediated T-cell activation.

Protocols

Product Specific Protocols
WB protocol for CARD11 antibody 28483-1-APDownload protocol
IHC protocol for CARD11 antibody 28483-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols