CARD14 Polyclonal antibody

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  WB analysis using 28373-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28373-1-AP (CARD14 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. Daudi cells were used as a negative control.

CARD14 Polyclonal Antibody for WB, ELISA

Cat No. 28373-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Synonyms

BIMP2, CARD14, Carma 2, CARMA2

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Tested Applications

• Positive WB detected in: EC109 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

28373-1-AP targets CARD14 in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag28969

  Product name: Recombinant human CARD14 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 280-566 aa of BC018142

  Sequence: SLAEKDILEQSLDEARGSRQELVERIHSLRERAVAAERQREQYWEEKEQTLLQFQKSKMACQLYREKVNALQAQVCELQKERDQAYSARDSAQREISQSLVEKDSLRRQVFELTDQVCELRTQLRQLQAEPPGVLKQEARTREPCPREKQRLVRMHAICPRDDSDCSLVSSTESQLLSDLSATSSRELVDSFRSSSPAPPSQQSLYKRVAEDFGEEPWSFSSCLEIPEGDPGALPGAKAGDPHLDYELLDTADLPQLESSLQPVSPGRLDVSESGVLMRRRPARRIL

• Full Name: caspase recruitment domain family, member 14
• Calculated Molecular Weight: 113 kDa
• Observed Molecular Weight: 110-140 kDa
• GenBank Accession Number: BC018142
• Gene Symbol: CARD14
• Gene ID (NCBI): 79092
• RRID: AB_3669656
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9BXL6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CARD14, also known as CARMA2, is closely related to CARD9, CARD10 , and CARD11 , which upon activation, each form an oligomeric CBM signaling complex with B‐cell lymphoma 10 (BCL10) and mucosa‐associated lymphoid tissue lymphoma translocation protein 1 (MALT1). It is predominantly expressed in epithelial cells of esophagus, skin, and mucosal tissues, although some isoforms were also shown to be expressed in hematopoietic cell lines.(PMID: 36009554, PMID: 32343482)