CARD8 Polyclonal antibody

CARD8 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 14516-1-AP

Synonyms

Apoptotic protein NDPP1, CARD8, CARDINAL, DACAR, Dakar, KIAA0955, NDPP, NDPP1, TUCAN



Tested Applications

Positive WB detected inA549 cells, HUVEC cells, THP-1 cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

14516-1-AP targets CARD8 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CARD8 fusion protein Ag5975
Full Name caspase recruitment domain family, member 8
Calculated molecular weight61 kDa, 49 kDa
Observed molecular weight61 kDa, 49 kDa
GenBank accession numberBC056891
Gene symbol CARD8
Gene ID (NCBI) 22900
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for CARD8 antibody 14516-1-APDownload protocol
IHC protocol for CARD8 antibody 14516-1-APDownload protocol
IF protocol for CARD8 antibody 14516-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols