CARD8 Polyclonal antibody

CARD8 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 14516-1-AP

Synonyms

Apoptotic protein NDPP1, CARD8, CARDINAL, DACAR, Dakar, KIAA0955, NDPP, NDPP1, TUCAN



Tested Applications

Positive WB detected inA549 cells, HUVEC cells, THP-1 cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

14516-1-AP targets CARD8 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CARD8 fusion protein Ag5975
Full Name caspase recruitment domain family, member 8
Calculated molecular weight61 kDa, 49 kDa
Observed molecular weight61 kDa, 49 kDa
GenBank accession numberBC056891
Gene symbol CARD8
Gene ID (NCBI) 22900
RRIDAB_2068262
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for CARD8 antibody 14516-1-APDownload protocol
IHC protocol for CARD8 antibody 14516-1-APDownload protocol
IF protocol for CARD8 antibody 14516-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols