CARD8 Polyclonal antibody

CARD8 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 14516-1-AP

Synonyms

Apoptotic protein NDPP1, CARD8, CARDINAL, DACAR, Dakar, KIAA0955, NDPP, NDPP1, TUCAN



Tested Applications

Positive WB detected inA549 cells, HUVEC cells, THP-1 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14516-1-AP targets CARD8 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CARD8 fusion protein Ag5975
Full Name caspase recruitment domain family, member 8
Calculated Molecular Weight61 kDa, 49 kDa
Observed Molecular Weight61 kDa, 49 kDa
GenBank Accession NumberBC056891
Gene Symbol CARD8
Gene ID (NCBI) 22900
RRIDAB_2068262
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CARD8 belongs to the caspase recruitment domain (CARD)-containing family of proteins, which are involved in pathways leading to activation of caspases or nuclear factor kappa-B (NFKB). CARD8 may be a component of the inflammasome, a protein complex that plays a role in the activation of proinflammatory caspases. It is thought that this protein acts as an adaptor molecule that negatively regulates NFKB activation, CASP1-dependent IL1B secretion, and apoptosis. Polymorphisms in this gene may be associated with a susceptibility to rheumatoid arthritis.

Protocols

Product Specific Protocols
WB protocol for CARD8 antibody 14516-1-APDownload protocol
IHC protocol for CARD8 antibody 14516-1-APDownload protocol
IF protocol for CARD8 antibody 14516-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols