CARD9 Polyclonal antibody

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CARD9 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 10669-1-AP Copied!

Publications (12)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Synonyms

Caspase recruitment domain-containing protein 9, hCARD9

• 

  WB analysis of THP-1 using 10669-1-AP

  WB result of CARD9 antibody (10669-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CARD9 transfected THP-1 cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HL-60 using 10669-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 10669-1-AP (CARD9 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse spleen using 10669-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 10669-1-AP (CARD9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HL-60 cells, THP-1 cells
• Positive IHC detected in: mouse spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

10669-1-AP targets CARD9 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag1057

  Product name: Recombinant human CARD9 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 193-492 aa of BC008877

  Sequence: SEEKGAALMRNRDLQLEIDQLKHSLMKAEDDCKVERKHTLKLRHAMEQRPSQELLWELQQEKALLQARVQELEASVQEGKLDRSSPYIQVLEEDWRQALRDHQEQANTIFSLRKDLRQGEARRLRCMEEKEMFELQCLALRKDSKMYKDRIEAILLQMEEVAIERDQAIATREELHAQHARGLQEKDALRKQVRELGEKADELQLQVFQCEAQLLAVEGRLRRQQLETLVLSSDLEDGSPRRSQELSLPQDLEDTQLSDKGCLAGGGSPKQPFAALHQEQVLRNPHDAGPAGLPGIGAVC

• Full Name: caspase recruitment domain family, member 9
• Calculated Molecular Weight: 62 kDa
• Observed Molecular Weight: 55-62 kDa
• GenBank Accession Number: BC008877
• Gene Symbol: CARD9
• Gene ID (NCBI): 64170
• RRID: AB_2228323
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9H257
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CARD9, a member of the caspase recruitment domain (CARD) protein family, plays an important regulatory role in cell apoptosis. Defects in CARD9 are the cause of familial candidiasis type 2 (CANDF2) characterized by impaired clearance of fungal infections and results in colonization and infections of the mucosa or skin, predominantly with Candida albicans. CARD9 is highly expressed in spleen and regulate activation of NF-kappa-B by interaction with postive regulator of apoptosis BCL10 via the CARD domain.

Publications

What published studies show

The CARD9 Polyclonal antibody (Cat# 10669-1-AP) has 12 citations published in journals including Blood, Phytomedicine, Frontiers in Immunology, JCI Insight, PLoS Pathogens, Journal of Cellular and Molecular Medicine, Translational Stroke Research, Journal of Clinical Immunology, and Plastic and Reconstructive Surgery. Research fields span CARD9 deficiency-related fungal infections, neuroinflammation and stroke via Dectin-1/TREM1 pathways, ovarian cancer progression through NF-κB activation, and cerebral ischemia-reperfusion injury.

Automatically generated from the 12 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Blood; Invasive fungal infection and impaired neutrophil killing in human CARD9 deficiency.; Authors - Drewniak Agata A
rat	WB	Phytomedicine; Rhubarb ameliorates ischemic stroke-induced tryptophan-kynurenine metabolic reprogramming and neuroinflammation via modulation of the IDO-1/TREM-1 pathway.; Authors - Xiaoyao Liu
mouse	WB	Phytomedicine; Jasminoidin and ursodeoxycholic acid exert synergistic effect against cerebral ischemia-reperfusion injury via Dectin-1-induced NF-κB activation pathway; Authors - Dan-Li Hao
mouse	WB	Front Immunol; TREM1 Regulates Neuroinflammatory Injury by Modulate Proinflammatory Subtype Transition of Microglia and Formation of Neutrophil Extracellular Traps via Interaction With SYK in Experimental Subarachnoid Hemorrhage.; Authors - Xinyan Wu
human	WB	JCI Insight; Extrapulmonary Aspergillus infection in patients with CARD9 deficiency.; Authors - Nikolaus Rieber
human	WB	PLoS Pathog; CARD9-Dependent Neutrophil Recruitment Protects against Fungal Invasion of the Central Nervous System.; Authors - Rebecca A Drummond