CARD9 Polyclonal antibody

• Featured Product
• KD/KO Validated

CARD9 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Publications

11

Cat no : 10669-1-AP

Synonyms

CARD9, hCARD9

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Validation Data Gallery

• 

  WB analysis of HL-60 using 10669-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 10669-1-AP (CARD9 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 10669-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 10669-1-AP (CARD9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HL-60 cells, PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 11 publications below
• IP: See 1 publications below

Product Information

10669-1-AP targets CARD9 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CARD9 fusion protein Ag1057
• Full Name: caspase recruitment domain family, member 9
• Calculated Molecular Weight: 62 kDa
• Observed Molecular Weight: 50-59 kDa
• GenBank Accession Number: BC008877
• Gene Symbol: CARD9
• Gene ID (NCBI): 64170
• RRID: AB_2228323
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CARD9, a member of the caspase recruitment domain (CARD) protein family, plays an important regulatory role in cell apoptosis. Defects in CARD9 are the cause of familial candidiasis type 2 (CANDF2) characterized by impaired clearance of fungal infections and results in colonization and infections of the mucosa or skin, predominantly with Candida albicans. CARD9 is highly expressed in spleen and regulate activation of NF-kappa-B by interaction with postive regulator of apoptosis BCL10 via the CARD domain.

Publications

Species	Application	Title
human	WB	Blood; Invasive fungal infection and impaired neutrophil killing in human CARD9 deficiency.; Authors - Drewniak Agata A
human	WB	PLoS Pathog; CARD9-Dependent Neutrophil Recruitment Protects against Fungal Invasion of the Central Nervous System.; Authors - Rebecca A Drummond
human	WB	J Clin Immunol; Inherited CARD9 Deficiency in a Child with Invasive Disease Due to Exophiala dermatitidis and Two Older but Asymptomatic Siblings.; Authors - Yusuke Imanaka
mouse	WB	Transl Stroke Res; Inhibition of Dectin-1 Ameliorates Neuroinflammation by Regulating Microglia/Macrophage Phenotype After Intracerebral Hemorrhage in Mice.; Authors - Xiongjie Fu
human	WB	Plast Reconstr Surg; Chronic Candida albicans Meningitis in a 4-Year-Old Girl with a Homozygous Mutation in the CARD9 Gene (Q295X).; Authors - Martin Herbst
mouse	WB	Front Immunol; TREM1 Regulates Neuroinflammatory Injury by Modulate Proinflammatory Subtype Transition of Microglia and Formation of Neutrophil Extracellular Traps via Interaction With SYK in Experimental Subarachnoid Hemorrhage.; Authors - Xinyan Wu