CARD9 Recombinant monoclonal antibody

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• CARD9 Recombinant monoclonal antibody (85749-1-RR)

CARD9 Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 85749-1-RR

Clone No.250045G3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

Caspase recruitment domain-containing protein 9, hCARD9

• 

  WB analysis using 85749-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85749-1-RR (CARD9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  Affinity of 85749-1-RR

  Biolayer interferometry (BLl) kinetic assays of 85749-1-RR against Human CARD9 were performed. The affinity constant is 14.3 nM.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HL-60 cells, RAW 264.7 cells, THP-1 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85749-1-RR targets CARD9 in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag33643

  Product name: Recombinant human CARD9 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 193-492 aa of BC008877

  Sequence: SEEKGAALMRNRDLQLEIDQLKHSLMKAEDDCKVERKHTLKLRHAMEQRPSQELLWELQQEKALLQARVQELEASVQEGKLDRSSPYIQVLEEDWRQALRDHQEQANTIFSLRKDLRQGEARRLRCMEEKEMFELQCLALRKDSKMYKDRIEAILLQMEEVAIERDQAIATREELHAQHARGLQEKDALRKQVRELGEKADELQLQVFQCEAQLLAVEGRLRRQQLETLVLSSDLEDGSPRRSQELSLPQDLEDTQLSDKGCLAGGGSPKQPFAALHQEQVLRNPHDAGPAGLPGIGAVC

• Full Name: caspase recruitment domain family, member 9
• Calculated Molecular Weight: 62 kDa
• Observed Molecular Weight: 60-65 kDa
• GenBank Accession Number: BC008877
• Gene Symbol: CARD9
• Gene ID (NCBI): 64170
• RRID: AB_3744343
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9H257
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CARD9, a member of the caspase recruitment domain (CARD) protein family, plays an important regulatory role in cell apoptosis. Defects in CARD9 are the cause of familial candidiasis type 2 (CANDF2), characterized by impaired clearance of fungal infections and resulting in colonization and infections of the mucosa or skin, predominantly with Candida albicans. CARD9 is highly expressed in the spleen and regulates activation of NF-kappa-B by interaction with the positive regulator of apoptosis BCL10 via the CARD domain.