CARHSP1 Polyclonal antibody

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  WB analysis using 11672-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11672-1-AP (CARHSP1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 11672-1-AP

  WB result of CARHSP1 antibody (11672-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CARHSP1 transfected HEK-293 cells.

• Featured Product
• KD/KO Validated

CARHSP1 Polyclonal Antibody for WB, ELISA

Cat No. 11672-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

Calcium-regulated heat-stable protein 1, Calcium-regulated heat-stable protein of 24 kDa, CRHSP 24, CRHSP-24, CSDC1

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Tested Applications

• Positive WB detected in: HEK-293 cells, mouse liver tissue, mouse testis tissue, rat liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

11672-1-AP targets CARHSP1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2265

  Product name: Recombinant human CARHSP1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-147 aa of BC003366

  Sequence: MSSEPPPPPQPPTHQASVGLLDTPRSRERSPSPLRGNVVPSPLPTRRTRTFSATVRASQGPVYKGVCKCFCRSKGHGFITPADGGPDIFLHISDVEGEYVPVEGDEVTYKMCSIPPKNEKLQAVEVVITHLAPGTKHETWSGHVISS

• Full Name: calcium regulated heat stable protein 1, 24kDa
• Calculated Molecular Weight: 147 aa, 16 kDa
• Observed Molecular Weight: 24 kDa
• GenBank Accession Number: BC003366
• Gene Symbol: CARHSP1
• Gene ID (NCBI): 23589
• RRID: AB_3669130
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9Y2V2
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CARHSP1(calcium-regulated heat-stable protein 1, a 24 kDa protein also known as CRHSP-24) is a ubiquitously expressed phosphoprotein that is comprised of 147 amino acids with nearly 14% proline (PMID: 21990353,30257675). CARHSP1 was discovered as a biomarker for diabetic retinopathy and as a regulator of TNF-α mRNA stability in macrophages (PMID: 21078874). CARHSP1 may be involved in oxidative stress via traffic between stress granules and processing bodies (PMID: 21177848).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Front Immunol; Development of a prognostic model for hepatocellular carcinoma based on microvascular invasion characteristic genes by spatial transcriptomics sequencing; Authors - Xiaolan Mu