CARM1 Polyclonal antibody

• Featured Product
• KD/KO Validated

CARM1 Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, monkey

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

11

Reviews (2)

Cat no : 55246-1-AP

Synonyms

CARM1, PRMT4

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Validation Data Gallery

• 

  WB analysis of NIH/3T3 using 55246-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 55246-1-AP (CARM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 55246-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 55246-1-AP (CARM1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 55246-1-AP

  WB result of CARM1 antibody (55246-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CARM1 transfected MCF-7 cells.

• 

  IHC staining of human breast cancer using 55246-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55246-1-AP (CARM1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 55246-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55246-1-AP (CARM1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human malignant melanoma using 55246-1-AP

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 55246-1-AP (CARM1 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human breast cancer using 55246-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 55246-1-AP (CARM1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 55246-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 55246-1-AP (CARM1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human cervical cancer using 55246-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 55246-1-AP (CARM1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 55246-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 55246-1-AP (CARM1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 55246-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55246-1-AP (CARM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 55246-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55246-1-AP (CARM1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 55246-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 55246-1-AP (CARM1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of NIH/3T3 using 55246-1-AP

  Immunofluorescent analysis of NIH/3T3 cells using 55246-1-AP (CARM1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive IHC detected in: human breast cancer tissue, human cervical cancer tissue, human malignant melanoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: NIH/3T3 cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 9 publications below
• IHC: See 2 publications below
• IF: See 3 publications below
• IP: See 1 publications below
• CoIP: See 2 publications below

Product Information

55246-1-AP targets CARM1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.

• Tested Reactivity: human, mouse, rat, monkey
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: coactivator-associated arginine methyltransferase 1
• Calculated Molecular Weight: 66 kDa
• Observed Molecular Weight: 63-66 kDa, 55 kDa
• GenBank Accession Number: NM_199141
• Gene Symbol: CARM1
• Gene ID (NCBI): 10498
• RRID: AB_10863291
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CARM1, also named as PRMT4, belongs to the protein arginine N-methyltransferase family. It is a dual functional coregulator that facilitates transcription initiation by methylation of Arg17 and Arg26 of histone H3 and also dictates the subsequent coactivator complex disassembly by methylation of the steroid receptor coactivator family coactivators and p300/cAMP-response element-binding protein. CARM1 functions as a coactivator for many nuclear receptors, such as oestrogen receptor, androgen receptor, thyroid receptor and farnesoid X-receptor. It also coactivates other transcription factors such as myocyte enhancer factor 2C (MEF2C), β-catenin, p53, nuclear factor (NF)-kB and the cAMP-responsive element-binding factor. The enzymatic activity and coactivator function of CARM1 has been found to be inactivated through phosphorylation at a conserved serine residue at mitosis stage. This antibody was generated against a synthetic peptide corresponding to a fragment of human CARM1. It is expected to specifically recognize the CRAM1. In certain type of cells, like Hela, double bands can be detected with this antibody. This may due to the additional PTM sites in cells themselves.

Publications

Species	Application	Title
human	WB	J Med Chem; Discovery of First-in-Class Dual PARP and EZH2 Inhibitors for Triple-Negative Breast Cancer with Wild-Type BRCA; Authors - Limei Ji
human	WB	Eur J Med Chem; Discovery of precision targeting EZH2 degraders for triple-negative breast cancer.; Authors - Cheng Wang
mouse	WB	Front Cell Dev Biol; Osteocytes Enhance Osteogenesis by Autophagy-Mediated FGF23 Secretion Under Mechanical Tension.; Authors - Huiyue Xu
human	WB,CoIP	Cancer Cell Int; CDCA4 suppresses epithelial-mesenchymal transtion (EMT) and metastasis in Non-small cell lung cancer through modulating autophagy.; Authors - Chenxin Xu ; KD Validated
human	WB	RSC Adv; Mechanistic insights into geniposide regulation of bile salt export pump (BSEP) expression; Authors - Guixin Wu
mouse	IF	J Reprod Immunol; Poly(I:C) exposure during in vitro fertilization disrupts first cleavage of mouse embryos and subsequent blastocyst development.; Authors - Zhicheng Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

fatemeh (Verified Customer) (07-25-2022)

western blot was done on 50 ug of cell lysate. Blot was transfered using wet transfer method (300 mA for 90 min). 5% milk was used as blocking reagent. antibody dilution was 1:1000 and incubation with primary antibody was overnight at 4C. Multiple band between 75 and 50 kDa were detected. for IF, PFA was used for fixing the cells and 0.5% triton was used for permeabilization. antibody was diluted 1:200 and samples were incubated with primary ab for 1h at RT. a cytoplasmic signal for Carm1 was detected.

• Applications: Western Blot, Immunofluorescence
• Cell Tissue Type: mESC

CARM1 Antibody Western Blot,Immunofluorescence validation ( dilution) in mESC (Cat no:55246-1-AP)

FH

Kyosuke (Verified Customer) (06-12-2019)

It works well on HUVECs for Western blot.

• Applications: Western Blot,
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: HUVECs