CARMIL Polyclonal antibody

• Home
• Products
• Primary Antibodies
• CARMIL Polyclonal antibody (27133-1-AP)

CARMIL Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 27133-1-AP Copied!

Publications (1)

Download datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, ELISA

Synonyms

CARMIL, CARMIL homolog, CARMIL1a, LRRC16, LRRC16A

• 

  WB analysis of MCF-7 using 27133-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 27133-1-AP (CARMIL antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 27133-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 27133-1-AP (CARMIL antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HeLa using 27133-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 27133-1-AP (CARMIL antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of MCF-7 using 27133-1-AP

  IP result of anti-CARMIL (IP:27133-1-AP, 4ug; Detection:27133-1-AP 1:1000) with MCF-7 cells lysate 2800 ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human colon using 27133-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27133-1-AP (CARMIL antibody at dilution of 1:200 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human colon using 27133-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27133-1-AP (CARMIL antibody at dilution of 1:200 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Able AI

What's the best dilution for CARMIL in ?

Chat with Able

---

Tested Applications

• Positive WB detected in: MCF-7 cells, HepG2 cells, HeLa cells
• Positive IP detected in: MCF-7 cells
• Positive IHC detected in: human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

27133-1-AP targets CARMIL in WB, IP, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag25876

  Product name: Recombinant human LRRC16A protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1021-1365 aa of BC140770

  Sequence: RVDEGVDEFFTKKVTKMDSKKWSTRGSESHELNEGGDEKKKRDSRKSSGFLNLIKSRSKSERPPTILMTEEPSSPKGAVRSPPVDCPRKDTKAAEHNGNSERIEEIKTPDSFEESQGEEIGKVERSDSKSSPQAGRRYGVQVMGSGLLAEMKAKQEKRAACAQKKLGNDAVSQDSSSPALSSVERSDGGGAGLPENRFGLGTPEKNTKAEPKAEAGSRSRSSSSTPTSPKPLLQSPKPSLAARPVIPQKPRTASRPDDIPDSPSSPKVALLPPVLKKVPSDKERDGQSSPQPSPRTFSQEVSRRSWGQQAQEYQEQKQRSSSKDGHQGSKSNDSGEEAEKEFIFV

• Full Name: leucine rich repeat containing 16A
• Observed Molecular Weight: 152 kDa
• GenBank Accession Number: BC140770
• Gene Symbol: CARMIL1
• Gene ID (NCBI): 55604
• RRID: AB_2880768
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q5VZK9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The CARMIL Polyclonal antibody (Cat# 27133-1-AP) has 1 total citation. It appears in International Immunopharmacology (2024), where it was used for IHC in human samples. The cited study investigated how CARMIL1 regulates liver cancer cell proliferation by activating the ERK/mTOR pathway through the TRIM27/p53 axis, placing this citation in the fields of oncology and signaling pathway research.

Automatically generated from the 1 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IHC	Int Immunopharmacol; CARMIL1 regulates liver cancer cell proliferation by activating the ERK/mTOR pathway through the TRIM27/p53 axis; Authors - Yuzhen Ge