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CARS Polyclonal antibody

CARS Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 15296-1-AP

Publications (4)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

CARS1, CYSRS, Cysteine tRNA ligase, Cysteine--tRNA ligase, cytoplasmic, cysteinyl tRNA synthetase

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

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Filter: AllWBIHCIF/ICC

WB analysis using 15296-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15296-1-AP (CARS antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of HeLa using 15296-1-AP
WB result of CARS antibody (15296-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CARS transfected HeLa cells.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse brain using 15296-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15296-1-AP (CARS antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse brain using 15296-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15296-1-AP (CARS antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human pancreas cancer using 15296-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 15296-1-AP (CARS antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human pancreas cancer using 15296-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 15296-1-AP (CARS antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of MCF-7 using 15296-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CARS antibody (15296-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
Publications (4)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	A549 cells, HeLa cells
Positive IHC detected in	mouse brain tissue, human pancreas cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	MCF-7 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunohistochemistry (IHC)	IHC : 1:250-1:1000
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15296-1-AP targets CARS in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag7546 Product name: Recombinant human CARS protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-353 aa of BC002880 Sequence: MADSSGQQGKGRRVQPQWSPPAGTQPCRLHLYNSLTRNKEVFIPQDGKKVTWYCCGPTVYDASHMGHARSYISFDILRRVLKDYFKFDVFYCMNITDIDDKIIKRARQNHLFEQYREKRPEAAQLLEDVQAALKPFSVKLNETTDPDKKQMLERIQHAVQLATEPLEKAVQSRLTGEEVNSCVEVLLEEAKDLLSDWLDSTLGCDVTDNSIFSKLPKFWEGDFHRDMEALNVLPPDVLTRVSEYVPEIVNFVQKIVDNGYGYVSNGSVYFDTAKFASSEKHSYGKLVPEAVGDQKALQEGEGDLSISADRLSEKRSPNDFALWKASKPGEPSWPCPWGKGRPGWHIECSAMAG Predict reactive species
Full Name	cysteinyl-tRNA synthetase
Calculated Molecular Weight	89 kDa
Observed Molecular Weight	70-85 kDa
GenBank Accession Number	BC002880
Gene Symbol	CARS
Gene ID (NCBI)	833
RRID	AB_2068455
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P49589
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IF protocol for CARS antibody 15296-1-AP	Download protocol
IHC protocol for CARS antibody 15296-1-AP	Download protocol
WB protocol for CARS antibody 15296-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The CARS polyclonal antibody (Cat# 15296-1-AP) has 4 total citations. These appear in Annals of the Rheumatic Diseases, EMBO Journal, Nitric Oxide, and Medicine (Baltimore). The associated research fields include antisynthetase autoantibody identification, cysteine metabolism and AMPK signaling, sepsis-induced acute kidney injury, and ferroptosis-related gene signatures in hepatocellular carcinoma.

Species	Application	Title
human	WB,IP	Ann Rheum Dis Mass spectrometry-based identification of new anti-Ly and known antisynthetase autoantibodies Authors - Jean-Baptiste Vulsteke View Article
human	WB	EMBO J CARS senses cysteine deprivation to activate AMPK for cell survival. Authors - Mengqiu Yuan KD Validated View Article
mouse	WB	Nitric Oxide H2S alleviated sepsis-induced acute kidney injury by inhibiting PERK/Bax-Bcl2 pathway Authors - Chengqing Song View Article
human	IHC	Medicine (Baltimore) Ferroptosis-related gene signature predicts prognosis and immunotherapy response in hepatocellular carcinoma: A multi-cohort retrospective study. Authors - Lipeng Li View Article