MLN51/CASC3 Polyclonal antibody

MLN51/CASC3 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 18047-1-AP

Synonyms

BTZ, CASC3, MLN 51, MLN51, Protein barentsz, Protein CASC3



Tested Applications

Positive WB detected inA2780 cells, MCF-7 cells, SKOV-3 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 18047-1-AP is MLN51/CASC3 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MLN51/CASC3 fusion protein Ag12694
Full Name cancer susceptibility candidate 3
Calculated Molecular Weight 703 aa, 76 kDa
Observed Molecular Weight 115-120 kDa
GenBank Accession NumberBC050526
Gene Symbol CASC3
Gene ID (NCBI) 22794
RRIDAB_10640534
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CASC3(cancer susceptibility candidate 3) is also named as MLN51 and belongs to the CASC3 family. It is induced by CSF2, and is important in the proliferation of RA fibroblast-like synoviocytes in the pathogenesis of rheumatoid arthritis (RA)(PMID:17101062). It is a nucleocytoplasmic protein containing, within its aminoterminal half, a coiled-coil domain followed by two nuclear localization signals (NLSs) responsible for nuclear localization and, in its carboxyl-terminal half, a nuclear export signal(NES) that mediates cytoplasmic retention. It possesses many potential phosphorylation sites and is found to be phosphorylated in both normal and cancer-derived cells.(PMID:17652158).

Protocols

Product Specific Protocols
WB protocol for MLN51/CASC3 antibody 18047-1-APDownload protocol
IHC protocol for MLN51/CASC3 antibody 18047-1-APDownload protocol
IF protocol for MLN51/CASC3 antibody 18047-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Cell

Identification of a microRNA that activates gene expression by repressing nonsense-mediated RNA decay.

Authors - Ivone G Bruno
humanWB,IF

Mol Biol Cell

Perispeckles are major assembly sites for the exon junction core complex.

Authors - Daguenet Elisabeth E