Tested Applications
| Positive WB detected in | Staurosporine treated Jurkat cells, HEK-293 cells, HeLa cells, mouse liver tissue |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10436-1-AP targets Caspase 2/P32/P18 in WB, IHC, IF, IP, RIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rabbit |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0694 Product name: Recombinant human Caspase 2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 18-217 aa of BC002427 Sequence: MAADRGRRILGVCGMHPHHQETLKKNRVVLAKQLLLSELLEHLLEKDIITLEMRELIQAKVGSFSQNVELLNLLPKRGPQAFDAFCEALRETKQGHLEDMLLTTLSGLQHVLPPLSCDYDLSLPFPVCESCPLYKKLRLSTDTVEHSLDNKDGPLCLQVKPCTPEFYQTHFQLAYRLQSRPRGLALVLSNVHFTGEKELE Predict reactive species |
| Full Name | caspase 2, apoptosis-related cysteine peptidase |
| Calculated Molecular Weight | 452 aa, 51 kDa |
| Observed Molecular Weight | 48-51 kDa, 32 kDa, 18kDa |
| GenBank Accession Number | BC002427 |
| Gene Symbol | Caspase 2 |
| Gene ID (NCBI) | 835 |
| RRID | AB_2228450 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P42575 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CASP2(Caspase-2) is also named as ICH1, NEDD2 and belongs to the peptidase C14A family.It is involved in the activation cascade of caspases responsible for apoptosis execution and might function by either activating some proteins required for cell death or inactivating proteins necessary for cell survival.It has 2 isoforms produced by alternative splicing and can exists almost as a dimer in solution(PMID:15865942).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for Caspase 2/P32/P18 antibody 10436-1-AP | Download protocol |
| IP protocol for Caspase 2/P32/P18 antibody 10436-1-AP | Download protocol |
| WB protocol for Caspase 2/P32/P18 antibody 10436-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Caspase 2/P32/P18 Polyclonal antibody (Cat# 10436-1-AP) has 13 citations published in journals including Nature Cell Biology, Journal of Clinical Investigation, Life Sciences, Food & Function, Frontiers in Microbiology, Frontiers in Pharmacology, Chemical Biology & Interactions, Experimental Neurology, Harmful Algae, International Journal of Medical Sciences, Brain Research, Toxicon, and Toxicology in Vitro. Research fields span apoptosis, necroptosis, protein quality control, hepatic metabolism, colorectal cancer, viral infection, stroke, and cellular toxicity mechanisms.
| Species | Application | Title |
|---|---|---|
Nat Cell Biol Caspase-2 is a condensate-mediated deubiquitinase in protein quality control | ||
J Clin Invest S100A11 regulates hepatic cholesterol metabolism and promotes steatohepatitis via non-canonical SREBP2 signaling | ||
Life Sci Timolol induces necroptosis, apoptosis and senescence concentration-dependently in rabbit Limbal stem cells in vitro. | ||
Food Funct Kaempferol exerts anti-colorectal cancer effects through its multi-target mediated glucose metabolism remodeling. | ||
Front Microbiol Enterovirus 71 Induces INF2 Cleavage via Activated Caspase-2 in Infected RD Cells. | ||
