Caspase 2/p32/p18 Polyclonal antibody

Caspase 2/p32/p18 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, RIP, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 10436-1-AP

Synonyms

CASP 2, CASP2, Caspase 2, Caspase 2/p18, Caspase2, ICH 1L, ICH 1L/1S, ICH1, NEDD 2, NEDD2, Protease ICH 1



Tested Applications

Positive WB detected inStaurosporine treated Jurkat cells, HEK-293 cells, HeLa cells, HL-60 cells, Jurkat cells, mouse kidney tissue, mouse liver tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB
Immunohistochemistry (IHC)IHC : 1:100-1:400
Sample-dependent, check data in validation data gallery

Product Information

10436-1-AP targets Caspase 2/p32/p18 in WB, RIP, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rabbit
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Caspase 2/p32/p18 fusion protein Ag0694
Full Name caspase 2, apoptosis-related cysteine peptidase
Calculated molecular weight 452 aa, 51 kDa
Observed molecular weight 51 kDa, 32 kDa, 18 kDa
GenBank accession numberBC002427
Gene symbol CASP2
Gene ID (NCBI) 835
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CASP2(Caspase-2) is also named as ICH1, NEDD2 and belongs to the peptidase C14A family.It is involved in the activation cascade of caspases responsible for apoptosis execution and might function by either activating some proteins required for cell death or inactivating proteins necessary for cell survival.It has 2 isoforms produced by alternative splicing and can exists almost as a dimer in solution(PMID:15865942).

Protocols

Product Specific Protocols
WB protocol for Caspase 2/p32/p18 antibody 10436-1-APDownload protocol
IHC protocol for Caspase 2/p32/p18 antibody 10436-1-APDownload protocol
IP protocol for Caspase 2/p32/p18 antibody 10436-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Brain Res

miR-1247-3p mediates apoptosis of cerebral neurons by targeting caspase-2 in stroke.

Authors - Rong Zhang
humanELISA

Toxicol In Vitro

PMID: 32320760

Authors - Ting Yang
rabbitRIP

Life Sci

Timolol induces necroptosis, apoptosis and senescence concentration-dependently in rabbit Limbal stem cells in vitro.

Authors - Guo-Jian Jiang

Front Pharmacol

PMID: 32210806

Authors - Wen Su
humanWB, IF

Front Microbiol

Enterovirus 71 Induces INF2 Cleavage via Activated Caspase-2 in Infected RD Cells.

Authors - Bei Wang