Validation Data Gallery
|Positive WB detected in
|Staurosporine treated Jurkat cells, HEK-293 cells, HeLa cells, HL-60 cells, Jurkat cells, mouse kidney tissue, mouse liver tissue
|Positive IP detected in
|Positive IHC detected in
|human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)
|WB : 1:500-1:1000
|IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
|IHC : 1:100-1:400
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
10436-1-AP targets Caspase 2/p32/p18 in WB, RIP, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
|human, mouse, rabbit
|Host / Isotype
|Rabbit / IgG
|Caspase 2/p32/p18 fusion protein Ag0694
|caspase 2, apoptosis-related cysteine peptidase
|Calculated molecular weight
|452 aa, 51 kDa
|Observed molecular weight
|48-51 kDa, 32 kDa, 18kDa
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
CASP2(Caspase-2) is also named as ICH1, NEDD2 and belongs to the peptidase C14A family.It is involved in the activation cascade of caspases responsible for apoptosis execution and might function by either activating some proteins required for cell death or inactivating proteins necessary for cell survival.It has 2 isoforms produced by alternative splicing and can exists almost as a dimer in solution(PMID:15865942).
|Product Specific Protocols
|WB protocol for Caspase 2/p32/p18 antibody 10436-1-AP
|IHC protocol for Caspase 2/p32/p18 antibody 10436-1-AP
|IP protocol for Caspase 2/p32/p18 antibody 10436-1-AP
|Click here to view our Standard Protocols
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