Tested Applications
| Positive WB detected in | Jurkat cells, HeLa cells, mouse spleen tissue, Staurosporine treated Jurkat cells, rat brain tissue, rat liver tissue |
| Positive IP detected in | NIH/3T3 cells |
| Positive IHC detected in | mouse brain tissue, human teeth tissue, human spleen tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse liver tissue, mouse brain tissue, mouse eye tissue |
| Positive IF/ICC detected in | NIH/3T3 cells, HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
19677-1-AP targets Caspase 3/P17/P19 in WB, IHC, IF/ICC, IF-P, IP, RIP, ELISA, Immunofluorescence, Western Blot applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, rabbit, monkey, chicken, zebrafish, sheep, goat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | caspase 3, apoptosis-related cysteine peptidase |
| Calculated Molecular Weight | 32 kDa |
| Observed Molecular Weight | 32-35 kDa, 17 kDa, 19 kDa |
| GenBank Accession Number | NM_004346 |
| Gene Symbol | Caspase 3 |
| Gene ID (NCBI) | 836 |
| RRID | AB_10733244 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P42574 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Caspases, a family of endoproteases, are critical players in cell regulatory networks controlling inflammation and cell death. Initiator caspases (caspase-2, -8, -9, -10, -11, and -12) cleave and activate downstream effector caspases (caspase-3, -6, and -7), which in turn execute apoptosis by cleaving targeted cellular proteins. Caspase 3 (also named CPP32, SCA-1, and Apopain) proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at the beginning of apoptosis. Caspase 3 plays a key role in the activation of sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Caspase 3 can also form heterocomplex with other proteins and performs the molecular mass of 50-70 kDa(PMID:9747872). This antibody can recognize p17, p19 and p32 of Caspase 3.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Caspase 3/P17/P19 antibody 19677-1-AP | Download protocol |
| IHC protocol for Caspase 3/P17/P19 antibody 19677-1-AP | Download protocol |
| IP protocol for Caspase 3/P17/P19 antibody 19677-1-AP | Download protocol |
| WB protocol for Caspase 3/P17/P19 antibody 19677-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Caspase 3/P17/P19 Polyclonal antibody (Cat# 19677-1-AP) has 3,138 total citations. These appear in high-impact journals including Nature Nanotechnology, Nature Cancer, Nature Communications, Nature Cell Biology, Cell Metabolism, Cell Research, Cancer Discovery, Molecular Cancer, Autophagy, Theranostics, Biomaterials, Cell Death & Differentiation, and Journal of Nanobiotechnology. Research fields span apoptosis and programmed cell death (pyroptosis, ferroptosis, PANoptosis), cancer biology and immunotherapy, nanomedicine/drug delivery, mitochondrial dysfunction, and organ injury (kidney, heart, liver).
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther Phase-separated nucleocapsid protein of SARS-CoV-2 suppresses cGAS-DNA recognition by disrupting cGAS-G3BP1 complex | ||
Mol Cancer Epigenetically upregulated oncoprotein PLCE1 drives esophageal carcinoma angiogenesis and proliferation via activating the PI-PLCε-NF-κB signaling pathway and VEGF-C/ Bcl-2 expression. | ||
Mol Cancer hsa_circ_0007919 induces LIG1 transcription by binding to FOXA1/TET1 to enhance the DNA damage response and promote gemcitabine resistance in pancreatic ductal adenocarcinoma | ||
Mol Cancer CMTM6 overexpression confers trastuzumab resistance in HER2-positive breast cancer | ||
Cell Metab RIPK1 senses S-adenosylmethionine scarcity to drive cell death and inflammation |
Reviews
What customers say
Users consistently rate this antibody 4–5 stars across 9 reviews. Western blot performance is highlighted most often, with clear, strong bands at dilutions of 1:500–1:1000 in various cell lines and tissues (kidney, heart, glioblastoma, NSC34, H69). One reviewer noted multiple bands in milk lysates but confirmed the most intense band matched the expected p32 caspase-3 fragment. It is also praised as a reliable apoptosis antibody for immunofluorescence. Overall, reviewers describe it as consistent, easy to use, and delivering satisfactory results every time.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Vignesh (Verified Customer) (01-28-2026) | Excellent
|
Charlotte (Verified Customer) (08-15-2024) | Done in milk. Many bands but the most intense one matches the p32 of the caspase 3
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Alessandro (Verified Customer) (12-09-2023) | Reliable apoptosis Ab for IF
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Azita (Verified Customer) (06-02-2021) | Western blot analysis using LC3B-Specific Polyclonal antibody in NSC34 cell line at dilution of 1:1000.
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Hala (Verified Customer) (04-12-2021) | works very well
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Isha (Verified Customer) (02-03-2021) | Gave me crystal clear bands in kidney lysates. really happy with the product
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Diane (Verified Customer) (02-02-2021) | Incubated overnight 4 degrees C. Secondary 1:2500. Excellent bands. Used Opti-4CN substrate kit for visualization.
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Chao (Verified Customer) (03-12-2020) | Full-length but not cleaved isoform is detected by western blot
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Kishor (Verified Customer) (01-30-2019) | It is an excellent antibody, worked every time when I used and got satisfactory results.
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