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Caspase 9/p35/p10 Polyclonal antibody
Caspase 9/p35/p10 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (7)
Applications
WB, RIP, IP, IHC, IF, FC, ELISA
Conjugate
Unconjugated
659
Cat no : 10380-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | Jurkat cells, HeLa cells, HL-60 cells, Staurosporine treated Jurkat cells |
Positive IP detected in | HeLa cells |
Positive IHC detected in | human heart tissue, mouse lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HepG2 cells |
Positive FC detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:300-1:1000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB |
Immunohistochemistry (IHC) | IHC : 1:50-1:200 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 1 publications below |
WB | See 629 publications below |
IHC | See 29 publications below |
IF | See 10 publications below |
ELISA | See 1 publications below |
RIP | See 1 publications below |
Product Information
10380-1-AP targets Caspase 9/p35/p10 in WB, RIP, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, goat, sheep, rabbit, hamster, pig, canine, bovine |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Caspase 9/p35/p10 fusion protein Ag0404 |
Full Name | caspase 9, apoptosis-related cysteine peptidase |
Calculated molecular weight | 46 kDa |
Observed molecular weight | 46 kDa, 30-39 kDa |
GenBank accession number | BC002452 |
Gene symbol | CASP9 |
Gene ID (NCBI) | 842 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Caspase 9, apoptosis-related cysteine protease (CASP9, synonyms: MCH6, APAF3, APAF-3, ICE-LAP6, CASPASE-9c)is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. Caspase 9 is processed by APAF1; this step is thought to be one of the earliest in the caspase activation cascade. 10380-1-AP can recognize the pre- and cleaved- caspase 9. In recent years, the localization of caspase 9 was a focus of interest. Beside its cytoplasmic distribution, a very extensive localization study was done on rat brain tissue, where caspase 9 was found located predominantly in the nucleus and to a lesser extend in the cytoplasm [PMID: 15541731].
Protocols
Product Specific Protocols | |
---|---|
WB protocol for Caspase 9/p35/p10 antibody 10380-1-AP | Download protocol |
IHC protocol for Caspase 9/p35/p10 antibody 10380-1-AP | Download protocol |
IF protocol for Caspase 9/p35/p10 antibody 10380-1-AP | Download protocol |
IP protocol for Caspase 9/p35/p10 antibody 10380-1-AP | Download protocol |
FC protocol for Caspase 9/p35/p10 antibody 10380-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Nat Commun Macrophage lineage cells-derived migrasomes activate complement-dependent blood-brain barrier damage in cerebral amyloid angiopathy mouse model | ||
Acta Pharm Sin B Miltirone induces cell death in hepatocellular carcinoma cell through GSDME-dependent pyroptosis. | ||
Carbohydr Polym A multi-bioresponsive self-assembled nano drug delivery system based on hyaluronic acid and geraniol against liver cancer | ||
Adv Healthc Mater A ROS-Responsive Liposomal Composite Hydrogel Integrating Improved Mitochondrial Function and Pro-Angiogenesis for Efficient Treatment of Myocardial Infarction. | ||
Haematologica Therapeutic effects of the novel subtype-selective histone deacetylase (HDAC) inhibitor chidamide on myeloma-associated bone disease. | ||
Clin Transl Med IGF2BP3 mediates the mRNA degradation of NF1 to promote triple-negative breast cancer progression via an m6A-dependent manner |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Nicola (Verified Customer) (04-11-2023) | Large amount of aspecific band on my western blot in the range 50-200 KDa.
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FH WEI (Verified Customer) (01-17-2020) | Good to detect apoptosis in mouse heart subject to IR injury.
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