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  • KD/KO Validated

Caspase 9/p35/p10 Polyclonal antibody

Caspase 9/p35/p10 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (7)

Applications

WB, RIP, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 10380-1-AP

Synonyms

APAF 3, APAF3, Apoptotic protease Mch 6, CASP 9, CASP9, Caspase 9, Caspase 9/p35/p10, CASPASE 9c, Caspase9, Caspase-9, ICE LAP6, ICE like apoptotic protease 6, MCH6



Tested Applications

Positive WB detected inJurkat cells, HeLa cells, HL-60 cells, Staurosporine treated Jurkat cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman heart tissue, mouse lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:300-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10380-1-AP targets Caspase 9/p35/p10 in WB, RIP, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, goat, sheep, rabbit, hamster, pig, canine, bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Caspase 9/p35/p10 fusion protein Ag0404
Full Name caspase 9, apoptosis-related cysteine peptidase
Calculated molecular weight 46 kDa
Observed molecular weight 46 kDa, 30-39 kDa
GenBank accession numberBC002452
Gene symbol CASP9
Gene ID (NCBI) 842
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Caspase 9, apoptosis-related cysteine protease (CASP9, synonyms: MCH6, APAF3, APAF-3, ICE-LAP6, CASPASE-9c)is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. Caspase 9 is processed by APAF1; this step is thought to be one of the earliest in the caspase activation cascade. 10380-1-AP can recognize the pre- and cleaved- caspase 9. In recent years, the localization of caspase 9 was a focus of interest. Beside its cytoplasmic distribution, a very extensive localization study was done on rat brain tissue, where caspase 9 was found located predominantly in the nucleus and to a lesser extend in the cytoplasm [PMID: 15541731].

Protocols

Product Specific Protocols
WB protocol for Caspase 9/p35/p10 antibody 10380-1-APDownload protocol
IHC protocol for Caspase 9/p35/p10 antibody 10380-1-APDownload protocol
IF protocol for Caspase 9/p35/p10 antibody 10380-1-APDownload protocol
IP protocol for Caspase 9/p35/p10 antibody 10380-1-APDownload protocol
FC protocol for Caspase 9/p35/p10 antibody 10380-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Nat Commun

Macrophage lineage cells-derived migrasomes activate complement-dependent blood-brain barrier damage in cerebral amyloid angiopathy mouse model

Authors - Mengyan Hu
humanWB

Acta Pharm Sin B

Miltirone induces cell death in hepatocellular carcinoma cell through GSDME-dependent pyroptosis.

Authors - Xiaowei Zhang
humanWB

Carbohydr Polym

A multi-bioresponsive self-assembled nano drug delivery system based on hyaluronic acid and geraniol against liver cancer

Authors - Shaofeng Duan
ratWB

Adv Healthc Mater

A ROS-Responsive Liposomal Composite Hydrogel Integrating Improved Mitochondrial Function and Pro-Angiogenesis for Efficient Treatment of Myocardial Infarction.

Authors - Zhi Zheng
WB

Haematologica

Therapeutic effects of the novel subtype-selective histone deacetylase (HDAC) inhibitor chidamide on myeloma-associated bone disease.

Authors - Jingsong He
humanWB

Clin Transl Med

IGF2BP3 mediates the mRNA degradation of NF1 to promote triple-negative breast cancer progression via an m6A-dependent manner

Authors - Xu Zhang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Nicola (Verified Customer) (04-11-2023)

Large amount of aspecific band on my western blot in the range 50-200 KDa.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Rat Neonatal Cardiomyocytes
FH

WEI (Verified Customer) (01-17-2020)

Good to detect apoptosis in mouse heart subject to IR injury.

  • Applications: Western Blot,
  • Cell Tissue Type: Mouse Heart