Caspase 9/p35/p10 Polyclonal antibody

• Featured Product
• KD/KO Validated

Caspase 9/p35/p10 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (7)

Applications

WB, RIP, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

659

Reviews (2)

Cat no : 10380-1-AP

Synonyms

APAF 3, APAF3, Apoptotic protease Mch 6, CASP 9, CASP9, Caspase 9, Caspase 9/p35/p10, CASPASE 9c, Caspase9, Caspase-9, ICE LAP6, ICE like apoptotic protease 6, MCH6

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Validation Data Gallery

• 

  WB analysis using 10380-1-AP

  Untreated and Staurosporine treated Jurkat cells were subjected to SDS PAGE followed by western blot with 10380-1-AP (Caspase 9/p35/p10 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 10380-1-AP

  Staurosporine treat Jurkat cells were subjected to SDS PAGE followed by western blot with 10380-1-AP (Caspase 9/p35/p10 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 10380-1-AP

  Staurosporine treated Jurkat cells were subjected to SDS PAGE followed by western blot with 10380-1-AP (Caspase 9/p35/p10 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10380-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10380-1-AP (Caspase 9/p35/p10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 10380-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 10380-1-AP (Caspase 9/p35/p10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 10380-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10380-1-AP (Caspase 9/p35/p10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 10380-1-AP

  IP result of anti-Caspase 9/p35/p10 (IP:10380-1-AP, 3ug; Detection:10380-1-AP 1:200) with HeLa cells lysate 2500 ug.

• 

  IP experiment of HeLa using 10380-1-AP

  IP Result of anti-Caspase 9/p35/p10 (IP:10380-1-AP, 4ug; Detection:10380-1-AP 1:500) with HeLa cells lysate 4000ug.

• 

  IHC staining of human heart using 10380-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 10380-1-AP (Caspase 9/p35/p10 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 10380-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 10380-1-AP (Caspase 9/p35/p10 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse lung using 10380-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 10380-1-AP (Caspase 9/p35/p10 antibody) at dilution of 1:25 (under 10x lens).

• 

  IHC staining of mouse lung using 10380-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 10380-1-AP (Caspase 9/p35/p10 antibody) at dilution of 1:25 (under 40x lens).

• 

  IF Staining of HepG2 using 10380-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 10380-1-AP (Caspase 9/p35/p10 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HepG2 using 10380-1-AP

  1X10^6 HepG2 cells were stained with 0.2ug Caspase 9/p35/p10 antibody (10380-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Tested Applications

• Positive WB detected in: Jurkat cells, HeLa cells, HL-60 cells, Staurosporine treated Jurkat cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human heart tissue, mouse lung tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells
• Positive FC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:300-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:50-1:200
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 629 publications below
• IHC: See 29 publications below
• IF: See 10 publications below
• ELISA: See 1 publications below
• RIP: See 1 publications below

Product Information

10380-1-AP targets Caspase 9/p35/p10 in WB, RIP, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, goat, sheep, rabbit, hamster, pig, canine, bovine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Caspase 9/p35/p10 fusion protein Ag0404
• Full Name: caspase 9, apoptosis-related cysteine peptidase
• Calculated molecular weight: 46 kDa
• Observed molecular weight: 46 kDa, 30-39 kDa
• GenBank accession number: BC002452
• Gene symbol: CASP9
• Gene ID (NCBI): 842
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Caspase 9, apoptosis-related cysteine protease (CASP9, synonyms: MCH6, APAF3, APAF-3, ICE-LAP6, CASPASE-9c)is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. Caspase 9 is processed by APAF1; this step is thought to be one of the earliest in the caspase activation cascade. 10380-1-AP can recognize the pre- and cleaved- caspase 9. In recent years, the localization of caspase 9 was a focus of interest. Beside its cytoplasmic distribution, a very extensive localization study was done on rat brain tissue, where caspase 9 was found located predominantly in the nucleus and to a lesser extend in the cytoplasm [PMID: 15541731].

Publications

Species	Application	Title
rat	WB	Nat Commun; Macrophage lineage cells-derived migrasomes activate complement-dependent blood-brain barrier damage in cerebral amyloid angiopathy mouse model; Authors - Mengyan Hu
human	WB	Acta Pharm Sin B; Miltirone induces cell death in hepatocellular carcinoma cell through GSDME-dependent pyroptosis.; Authors - Xiaowei Zhang
human	WB	Carbohydr Polym; A multi-bioresponsive self-assembled nano drug delivery system based on hyaluronic acid and geraniol against liver cancer; Authors - Shaofeng Duan
rat	WB	Adv Healthc Mater; A ROS-Responsive Liposomal Composite Hydrogel Integrating Improved Mitochondrial Function and Pro-Angiogenesis for Efficient Treatment of Myocardial Infarction.; Authors - Zhi Zheng
	WB	Haematologica; Therapeutic effects of the novel subtype-selective histone deacetylase (HDAC) inhibitor chidamide on myeloma-associated bone disease.; Authors - Jingsong He
human	WB	Clin Transl Med; IGF2BP3 mediates the mRNA degradation of NF1 to promote triple-negative breast cancer progression via an m6A-dependent manner; Authors - Xu Zhang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Nicola (Verified Customer) (04-11-2023)

Large amount of aspecific band on my western blot in the range 50-200 KDa.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Rat Neonatal Cardiomyocytes

FH

WEI (Verified Customer) (01-17-2020)

Good to detect apoptosis in mouse heart subject to IR injury.

• Applications: Western Blot,
• Cell Tissue Type: Mouse Heart

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