Caspase 9/P35/P10 Polyclonal antibody

• 

  WB analysis using 10380-1-AP

  Untreated and Staurosporine treated Jurkat cells were subjected to SDS PAGE followed by western blot with 10380-1-AP (Caspase 9/P35/P10 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 10380-1-AP

  Staurosporine treat Jurkat cells were subjected to SDS PAGE followed by western blot with 10380-1-AP (Caspase 9/P35/P10 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 10380-1-AP

  Staurosporine treated Jurkat cells were subjected to SDS PAGE followed by western blot with 10380-1-AP (Caspase 9/P35/P10 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 10380-1-AP

  IP result of anti-Caspase 9/P35/P10 (IP:10380-1-AP, 3ug; Detection:10380-1-AP 1:200) with HeLa cells lysate 2500 ug.

• 

  IP experiment of HeLa using 10380-1-AP

  IP result of anti-Caspase 9/P35/P10 (IP:10380-1-AP, 4ug; Detection:10380-1-AP 1:500) with HeLa cells lysate 4000ug.

• 

  IHC staining of mouse lung using 10380-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 10380-1-AP (Caspase 9/P35/P10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 10380-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 10380-1-AP (Caspase 9/P35/P10 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 10380-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 10380-1-AP (Caspase 9/P35/P10 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 10380-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 10380-1-AP (Caspase 9/P35/P10 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse lung using 10380-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 10380-1-AP (Caspase 9/P35/P10 antibody) at dilution of 1:25 (under 10x lens).

• 

  IHC staining of mouse lung using 10380-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 10380-1-AP (Caspase 9/P35/P10 antibody) at dilution of 1:25 (under 40x lens).

• 

  IF Staining of HepG2 using 10380-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Caspase 9/P35/P10 antibody (10380-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

• Featured Product
• KD/KO Validated

Caspase 9/P35/P10 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 10380-1-AP

Publications(822)

Reviews (3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (8)

Applications

WB, IHC, IF/ICC, IP, RIP, ELISA

Synonyms

CASP9, Caspase 9, APAF 3, APAF3, APAF-3

---

Tested Applications

• Positive WB detected in: Jurkat cells, Staurosporine treated Jurkat cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: mouse lung tissue, human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:300-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 786 publications below
• IHC: See 38 publications below
• IF: See 15 publications below
• ELISA: See 1 publications below
• RIP: See 1 publications below

Product Information

10380-1-AP targets Caspase 9/P35/P10 in WB, IHC, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat, pig, rabbit, chicken, zebrafish, hamster, sheep, goat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Caspase 9/P35/P10 fusion protein Ag0404
• Full Name: caspase 9, apoptosis-related cysteine peptidase
• Calculated Molecular Weight: 46 kDa
• Observed Molecular Weight: 46 kDa, 30-39 kDa
• GenBank Accession Number: BC002452
• Gene Symbol: Caspase 9
• Gene ID (NCBI): 842
• RRID: AB_2068632
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P55211
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Caspase 9, apoptosis-related cysteine protease (CASP9, synonyms: MCH6, APAF3, APAF-3, ICE-LAP6, CASPASE-9c)is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. Caspase 9 is processed by APAF1; this step is thought to be one of the earliest in the caspase activation cascade. 10380-1-AP can recognize the pre- and cleaved- caspase 9. In recent years, the localization of caspase 9 was a focus of interest. Beside its cytoplasmic distribution, a very extensive localization study was done on rat brain tissue, where caspase 9 was found located predominantly in the nucleus and to a lesser extend in the cytoplasm [PMID: 15541731].

Publications

Species	Application	Title
human	WB	Mol Cancer; hsa_circ_0007919 induces LIG1 transcription by binding to FOXA1/TET1 to enhance the DNA damage response and promote gemcitabine resistance in pancreatic ductal adenocarcinoma; Authors - Lei Xu
mouse	WB,IHC	Bioact Mater; Silicate ions as soluble form of bioactive ceramics alleviate aortic aneurysm and dissection; Authors - Yumei Que
rat	WB	Nat Commun; Macrophage lineage cells-derived migrasomes activate complement-dependent blood-brain barrier damage in cerebral amyloid angiopathy mouse model; Authors - Mengyan Hu
human	WB	Acta Pharm Sin B; Miltirone induces cell death in hepatocellular carcinoma cell through GSDME-dependent pyroptosis.; Authors - Xiaowei Zhang
human	WB	Carbohydr Polym; A multi-bioresponsive self-assembled nano drug delivery system based on hyaluronic acid and geraniol against liver cancer; Authors - Shaofeng Duan
rat	WB	Adv Healthc Mater; A ROS-Responsive Liposomal Composite Hydrogel Integrating Improved Mitochondrial Function and Pro-Angiogenesis for Efficient Treatment of Myocardial Infarction.; Authors - Zhi Zheng

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Christoffer (Verified Customer) (03-03-2025)

Works well on adult mouse retina embedded in paraffin as well as cryo embedded postnatal (p7) mouse tissue.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1/200
• Cell Tissue Type: Adult mouse retina, Embryonic (E14) mouse retina.

FH

Nicola (Verified Customer) (04-11-2023)

Large amount of aspecific band on my western blot in the range 50-200 KDa.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Rat Neonatal Cardiomyocytes

FH

WEI (Verified Customer) (01-17-2020)

Good to detect apoptosis in mouse heart subject to IR injury.

• Applications: Western Blot,
• Cell Tissue Type: Mouse Heart