CASPR Polyclonal antibody

CASPR Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 55417-1-AP

Synonyms

CNTNAP1, Contactin-associated protein 1, contactin associated protein 1

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Validation Data Gallery

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  WB analysis of mouse brain using 55417-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55417-1-AP (CASPR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human cerebellum using 55417-1-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum using 55417-1-AP (CASPR antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human cerebellum using 55417-1-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum using 55417-1-AP (CASPR antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of mouse cerebellum using 55417-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using CASPR antibody (55417-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse brain tissue
• Positive IHC detected in: human cerebellum tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse cerebellum tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

55417-1-AP targets CASPR in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: contactin associated protein 1
• Calculated Molecular Weight: 156 kDa
• Observed Molecular Weight: 180-190 kDa
• GenBank Accession Number: NM_003632
• Gene Symbol: CASPR
• Gene ID (NCBI): 8506
• RRID: AB_11232211
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CNTNAP1, also named as CASPR, NRXN4, p190, Neurexin IV and Neurexin-4, belongs to the neurexin family. It plays a role in the formation of functional distinct domains critical for saltatory conduction of nerve impulses in myelinated nerve fibers. CNTNAP1 seems to demarcate the paranodal region of the axo-glial junction. In association with contactin, CNTNAP1 may have a role in the signaling between axons and myelinating glial cells. This antibody is specific to CNTNAP1.

Publications

Species	Application	Title
mouse	IF	Front Psychiatry; Analysis of the influences of social isolation on cognition and the therapeutic potential of deep brain stimulation in a mouse model; Authors - Yun-Yun Hu