Tested Applications
| Positive WB detected in | mouse brain tissue, rat brain tissue |
| Positive IHC detected in | mouse cerebellum tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse cerebellum tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
55417-1-AP targets CASPR in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | contactin associated protein 1 |
| Calculated Molecular Weight | 156 kDa |
| Observed Molecular Weight | 180-190 kDa |
| GenBank Accession Number | NM_003632 |
| Gene Symbol | CASPR |
| Gene ID (NCBI) | 8506 |
| RRID | AB_11232211 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P78357 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CNTNAP1, also named as CASPR, NRXN4, p190, Neurexin IV and Neurexin-4, belongs to the neurexin family. It plays a role in the formation of functional distinct domains critical for saltatory conduction of nerve impulses in myelinated nerve fibers. CNTNAP1 seems to demarcate the paranodal region of the axo-glial junction. In association with contactin, CNTNAP1 may have a role in the signaling between axons and myelinating glial cells. This antibody is specific to CNTNAP1.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CASPR antibody 55417-1-AP | Download protocol |
| IHC protocol for CASPR antibody 55417-1-AP | Download protocol |
| WB protocol for CASPR antibody 55417-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CASPR Polyclonal antibody (Cat# 55417-1-AP) has 4 citations. They appear in Advanced Science, Journal of Neuroinflammation, Acta Pharmacologica Sinica, and Frontiers in Psychiatry. Research fields include white matter stroke repair, lysosomal cholesteryl ester hydrolysis in microglia, cognitive dysfunction following subarachnoid hemorrhage, and social isolation/deep brain stimulation in mouse models.
| Species | Application | Title |
|---|---|---|
Adv Sci (Weinh) Inhibition of SLC11A1-Mediated Lysosomal Iron Accumulation in Microglia Promotes Repair Following White Matter Stroke. | ||
J Neuroinflammation Lysosomal cholesteryl ester hydrolysis drives white matter repair by reprogramming microglia into a novel reparative state. | ||
Acta Pharmacol Sin Clemastine alleviates cognitive dysfunction after SAH by targeting neuronal CNTN1 to regulate the ADA-adenosine pathway. | ||
Front Psychiatry Analysis of the influences of social isolation on cognition and the therapeutic potential of deep brain stimulation in a mouse model |
Reviews
What customers say
The single review for this product reports a 4-star rating from an IHC application. The user used a 1:500 dilution on PFA-fixed cryosectioned mouse brain tissue and found it worked well with minimal background noise. Overall, the feedback is positive, highlighting clean staining and low background in immunohistochemistry.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Amy (Verified Customer) (09-03-2026) | Worked well on cryo mice brain tissus fixed with PFA4%. Not a lot of background noise.
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