CBFA2T3 Polyclonal antibody

• Featured Product
• KD/KO Validated

CBFA2T3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ChIP, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 17190-1-AP

Synonyms

MTG8-related protein 2, MTG8 related protein 2, MTG16, hMTG16, ETO2

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Validation Data Gallery

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  WB analysis of Jurkat using 17190-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 17190-1-AP (CBFA2T3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 17190-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 17190-1-AP (CBFA2T3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 17190-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 17190-1-AP (CBFA2T3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of rat brain using 17190-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 17190-1-AP (CBFA2T3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: Jurkat cells
• Positive IHC detected in: rat brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• ChIP: See 1 publications below

Product Information

17190-1-AP targets CBFA2T3 in WB, IHC, IF, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CBFA2T3 fusion protein Ag9265
• Full Name: core-binding factor, runt domain, alpha subunit 2; translocated to, 3
• Calculated Molecular Weight: 71 kDa
• Observed Molecular Weight: 62-65 kDa
• GenBank Accession Number: BC062624
• Gene Symbol: CBFA2T3
• Gene ID (NCBI): 863
• RRID: AB_10859097
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB, IHC, IF	Oncotarget; miR-27a and miR-27a* contribute to metastatic properties of osteosarcoma cells.; Authors - Zaidoun Salah
human,mouse	WB,IHC	JCI Insight; MTG16 is a tumor suppressor in colitis-associated carcinoma.; Authors - Elizabeth M McDonough ; KO Validated
mouse	WB,ChIP	J Biol Chem; Transcription factor Zbtb1 interacts with bridging factor Lmo2 and maintains the T-lineage differentiation capacity of lymphoid progenitor cells; Authors - Maria Koizumi
		Leukemia; High caspase 3 and vulnerability to dual BCL2 family inhibition define ETO2::GLIS2 pediatric leukemia; Authors - Zakia Aid