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- KD/KO Validated
c-Cbl Polyclonal antibody
c-Cbl Polyclonal Antibody for IHC, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IP, IHC, IF, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 25818-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | K-562 cells, Raji cells, Jurkat cells, Ramos cells, Romas cells |
Positive IP detected in | K-562 cells |
Positive IHC detected in | human lymphoma tissue, human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 3 publications below |
WB | See 11 publications below |
IF | See 3 publications below |
IP | See 3 publications below |
CoIP | See 2 publications below |
Product Information
The immunogen of 25818-1-AP is c-Cbl Fusion Protein expressed in E. coli.
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | c-Cbl fusion protein Ag23033 |
Full Name | Cas-Br-M (murine) ecotropic retroviral transforming sequence |
Calculated molecular weight | 906 aa, 100 kDa |
Observed molecular weight | 100-120 kDa |
GenBank accession number | BC132733 |
Gene symbol | CBL |
Gene ID (NCBI) | 867 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
Product Specific Protocols | |
---|---|
WB protocol for c-Cbl antibody 25818-1-AP | Download protocol |
IHC protocol for c-Cbl antibody 25818-1-AP | Download protocol |
IP protocol for c-Cbl antibody 25818-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Cell Rep Pro-prion, as a membrane adaptor protein for E3 ligase c-Cbl, facilitates the ubiquitination of IGF-1R, promoting melanoma metastasis
| ||
Cell Death Dis ZC3H15 promotes glioblastoma progression through regulating EGFR stability. | ||
Cell Commun Signal Gasdermin E regulates the stability and activation of EGFR in human non-small cell lung cancer cells | ||
J Control Release Imatinib co-loaded targeted realgar nanocrystal for synergistic therapy of chronic myeloid leukemia. | ||
Oxid Med Cell Longev Salidroside-Mediated Autophagic Targeting of Active Src and Caveolin-1 Suppresses Low-Density Lipoprotein Transcytosis across Endothelial Cells. |