• Featured Product
  • KD/KO Validated

c-Cbl Polyclonal antibody

c-Cbl Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 25818-1-AP

Synonyms

C CBL, CBL, CBL2, c-Cbl, Proto oncogene c Cbl, RING finger protein 55, RNF55



Tested Applications

Positive WB detected inK-562 cells, Raji cells, Jurkat cells, Ramos cells, Romas cells
Positive IP detected inK-562 cells
Positive IHC detected inhuman lymphoma tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25818-1-AP targets c-Cbl in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen c-Cbl fusion protein Ag23033
Full Name Cas-Br-M (murine) ecotropic retroviral transforming sequence
Calculated Molecular Weight 906 aa, 100 kDa
Observed Molecular Weight 100-120 kDa
GenBank Accession NumberBC132733
Gene Symbol CBL
Gene ID (NCBI) 867
RRIDAB_2880252
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

c-Cbl (Casitas B-lineage lymphoma) is a critical regulator that controls immature or follicular helper T cell development, B cell development, immune tolerance, angiogenesis, or tumorigenesis (PMID: 33962939). c-Cbl also plays a pivotal role in regulating dendritic cell (DC) function via negatively modulating IL-12 production after Toll-like receptor (TLR) stimulation (PMID: 21799517).

Protocols

Product Specific Protocols
WB protocol for c-Cbl antibody 25818-1-APDownload protocol
IHC protocol for c-Cbl antibody 25818-1-APDownload protocol
IP protocol for c-Cbl antibody 25818-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Hepatology

PMID: 29729186

Authors - Huixia Zhang
  • KD Validated
humanWB,IF,CoIP

Cell Rep

Pro-prion, as a membrane adaptor protein for E3 ligase c-Cbl, facilitates the ubiquitination of IGF-1R, promoting melanoma metastasis

Authors - Huan Li
  • KD Validated
humanWB

Cell Death Dis

ZC3H15 promotes glioblastoma progression through regulating EGFR stability.

Authors - Jianbing Hou
humanWB

Cell Commun Signal

Gasdermin E regulates the stability and activation of EGFR in human non-small cell lung cancer cells

Authors - Limei Xu
humanWB

Oncogene

AKIP1 accelerates glioblastoma progression through stabilizing EGFR expression

Authors - Sicheng Wan
humanWB

J Control Release

Imatinib co-loaded targeted realgar nanocrystal for synergistic therapy of chronic myeloid leukemia.

Authors - Shengmei Wang