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c-Cbl Monoclonal antibody
c-Cbl Monoclonal Antibody for IHC, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human and More (1)
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
CloneNo.
2F6D1
Cat no : 66576-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HL-60 cells, Daudi cells, K-562 cells, Jurkat cells, THP-1 cells, Raji cells |
Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:300-1:1200 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 1 publications below |
WB | See 2 publications below |
Product Information
66576-1-Ig targets c-Cbl in WB, IHC, ELISA applications and shows reactivity with Human samples.
Tested Reactivity | Human |
Cited Reactivity | human |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | c-Cbl fusion protein Ag23033 |
Full Name | Cas-Br-M (murine) ecotropic retroviral transforming sequence |
Calculated molecular weight | 906 aa, 100 kDa |
Observed molecular weight | 100-120 kDa |
GenBank accession number | BC132733 |
Gene symbol | CBL |
Gene ID (NCBI) | 867 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
Product Specific Protocols | |
---|---|
WB protocol for c-Cbl antibody 66576-1-Ig | Download protocol |
IHC protocol for c-Cbl antibody 66576-1-Ig | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Autophagy RAB26-dependent autophagy protects adherens junctional integrity in acute lung injury.
| ||
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Kus (Verified Customer) (12-08-2022) | This Ab always worked perfect for our experiments in western blotting at 1:1000 or 2000 dilution in 5% BSA+TBST.
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