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c-Cbl Monoclonal antibody

c-Cbl Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (1)

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

CloneNo.

2F6D1

Cat no : 66576-1-Ig

Synonyms

C CBL, CBL, CBL2, c-Cbl, Proto oncogene c Cbl, RING finger protein 55, RNF55



Tested Applications

Positive WB detected inHL-60 cells, Daudi cells, K-562 cells, Jurkat cells, THP-1 cells, Raji cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Sample-dependent, check data in validation data gallery

Product Information

66576-1-Ig targets c-Cbl in WB, IHC,ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen c-Cbl fusion protein Ag23033
Full Name Cas-Br-M (murine) ecotropic retroviral transforming sequence
Calculated molecular weight 906 aa, 100 kDa
Observed molecular weight 100-120 kDa
GenBank accession numberBC132733
Gene symbol CBL
Gene ID (NCBI) 867
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for c-Cbl antibody 66576-1-IgDownload protocol
IHC protocol for c-Cbl antibody 66576-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Autophagy

RAB26-dependent autophagy protects adherens junctional integrity in acute lung injury.

Authors - Weijie Dong
  • KD Validated