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  • KD/KO Validated

c-Cbl Monoclonal antibody

c-Cbl Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2F6D1

Cat no : 66576-1-Ig

Synonyms

C CBL, CBL, CBL2, c-Cbl, Proto oncogene c Cbl, RING finger protein 55, RNF55



Tested Applications

Positive WB detected inHL-60 cells, Daudi cells, K-562 cells, Jurkat cells, THP-1 cells, Raji cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

66576-1-Ig targets c-Cbl in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen c-Cbl fusion protein Ag23033
Full Name Cas-Br-M (murine) ecotropic retroviral transforming sequence
Calculated molecular weight 906 aa, 100 kDa
Observed molecular weight 100-120 kDa
GenBank accession numberBC132733
Gene symbol CBL
Gene ID (NCBI) 867
RRIDAB_2881936
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

c-Cbl (Casitas B-lineage lymphoma) is a critical regulator that controls immature or follicular helper T cell development, B cell development, immune tolerance, angiogenesis, or tumorigenesis (PMID: 33962939). c-Cbl also plays a pivotal role in regulating dendritic cell (DC) function via negatively modulating IL-12 production after Toll-like receptor (TLR) stimulation (PMID: 21799517).

Protocols

Product Specific Protocols
WB protocol for c-Cbl antibody 66576-1-IgDownload protocol
IHC protocol for c-Cbl antibody 66576-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Autophagy

RAB26-dependent autophagy protects adherens junctional integrity in acute lung injury.

Authors - Weijie Dong
  • KD Validated
humanWB

Cytokine

The CBL-LSD1-CXCL8 axis regulates methionine metabolism in glioma.

Authors - Jie Chang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Kus (Verified Customer) (12-08-2022)

This Ab always worked perfect for our experiments in western blotting at 1:1000 or 2000 dilution in 5% BSA+TBST.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Human Liver cells
c-Cbl Antibody Western Blot validation (1:1000 dilution) in Human Liver cells (Cat no:66576-1-Ig)