Tested Applications
| Positive WB detected in | HL-60 cells, Daudi cells, K-562 cells, Jurkat cells, THP-1 cells, Raji cells |
| Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human tonsillitis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:300-1:1200 |
| Immunofluorescence (IF)-P | IF-P : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66576-1-Ig targets c-Cbl in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag23033 Product name: Recombinant human CBL protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 578-906 aa of BC132733 Sequence: LPPVPSSRLGDSWLPRPIPKVPVSAPSSSDPWTGRELTNRHSLPFSLPSQMEPRPDVPRLGSTFSLDTSMSMNSSPLVGPECDHPKIKPSSSANAIYSLAARPLPVPKLPPGEQCEGEEDTEYMTPSSRPLRPLDTSQSSRACDCDQQIDSCTYEAMYNIQSQAPSITESSTFGEGNLAAAHANTGPEESENEDDGYDVPKPPVPAVLARRTLSDISNASSSFGWLSLDGDPTTNVTEGSQVPERPPKPFPRRINSERKAGSCQQGSGPAASAATASPQLSSEIENLMSQGYSYQDIQKALVIAQNNIEMAKNILREFVSISSPAHVAT Predict reactive species |
| Full Name | Cas-Br-M (murine) ecotropic retroviral transforming sequence |
| Calculated Molecular Weight | 906 aa, 100 kDa |
| Observed Molecular Weight | 100-120 kDa |
| GenBank Accession Number | BC132733 |
| Gene Symbol | CBL |
| Gene ID (NCBI) | 867 |
| RRID | AB_2881936 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P22681 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
c-Cbl (Casitas B-lineage lymphoma) is a critical regulator that controls immature or follicular helper T cell development, B cell development, immune tolerance, angiogenesis, or tumorigenesis (PMID: 33962939). c-Cbl also plays a pivotal role in regulating dendritic cell (DC) function via negatively modulating IL-12 production after Toll-like receptor (TLR) stimulation (PMID: 21799517).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for c-Cbl antibody 66576-1-Ig | Download protocol |
| IHC protocol for c-Cbl antibody 66576-1-Ig | Download protocol |
| WB protocol for c-Cbl antibody 66576-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The c-Cbl Monoclonal antibody (2F6D1), catalog number 66576-1-Ig, has 2 total citations. These appear in Autophagy (2018), studying RAB26-dependent autophagy in acute lung injury, and Cytokine (2022), investigating the CBL-LSD1-CXCL8 axis in glioma methionine metabolism. The associated research fields include autophagy, lung injury, glioma, and cytokine signaling. Both citations used the antibody for Western blot in human samples.
| Species | Application | Title |
|---|---|---|
Autophagy RAB26-dependent autophagy protects adherens junctional integrity in acute lung injury.
| ||
Reviews
What customers say
One reviewer (Kus Pung) gave this antibody a 5-star rating for Western Blot on human liver cells using lot 10005438 at a 1:1000 dilution in 5% BSA/TBST. They reported consistent, perfect performance across their experiments, noting it also works well at 1:2000 dilution. Overall, the feedback highlights reliable and reproducible WB results with this reagent.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Kus (Verified Customer) (12-08-2022) | This Ab always worked perfect for our experiments in western blotting at 1:1000 or 2000 dilution in 5% BSA+TBST.
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