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CoraLite®488-conjugated CBP,EIF4E Polyclonal antibody

CBP,EIF4E Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra)

Conjugate

CoraLite®488 Fluorescent Dye

Cat no : CL488-11149

Synonyms

CBP, eIF 4E, eIF 4F 25 kDa subunit, EIF4E, EIF4E1, EIF4EL1, EIF4F, mRNA cap binding protein



Tested Applications

Positive FC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 11149-1-AP

Recommended dilution

ApplicationDilution
Sample-dependent, check data in validation data gallery

Product Information

CL488-11149 targets CBP,EIF4E in FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CBP,EIF4E fusion protein Ag1626
Full Name eukaryotic translation initiation factor 4E
Calculated molecular weight 29 kDa
Observed molecular weight 26-29 kDa, 50 kDa
GenBank accession numberBC012611
Gene symbol EIF4E
Gene ID (NCBI) 1977
Conjugate CoraLite®488 Fluorescent Dye
Excitation/Emission maxima wavelengths488 nm / 515 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

Eukaryotic translation initiation factor 4E(EIF4E) is the rate-limiting part of mRNA 7-methyl guanosine cap-binding component of the translation initiation complex eIF4F. The other subunits of EIF4F are a 50-kD polopeptied, terned EIF4A and a 220-kD polypeptied EIF4G. EIF4E mediates the binding to the mRNA cap then repress the translation. Due to the increasing expression of c-Myc in the oncogene, EIF4E's activity is enhance. This is a rabbit polyclonal antibody raised against the full-length of human EIF4E. This protein can form a dimer (~50 kDa).

Protocols

Product Specific Protocols
FC protocol for CL488 CBP,EIF4E antibody CL488-11149Download protocol
Standard Protocols
Click here to view our Standard Protocols