Validation Data Gallery
|Positive WB detected in||HeLa cells, HEK-293 cells, HEK-293T cells, HepG2 cells, Jurkat cells, K-562 cells|
|Positive IHC detected in||human breast cancer tissue, human gliomas tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Positive FC detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
11149-1-AP targets EIF4E in WB, IHC, IF, FC, ELISA applications and shows reactivity with human samples.
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||EIF4E fusion protein Ag1626|
|Full Name||eukaryotic translation initiation factor 4E|
|Calculated molecular weight||29 kDa|
|Observed molecular weight||26-29 kDa, 50 kDa|
|GenBank accession number||BC012611|
|Gene ID (NCBI)||1977|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Eukaryotic translation initiation factor 4E(EIF4E) is the rate-limiting part of mRNA 7-methyl guanosine cap-binding component of the translation initiation complex eIF4F. The other subunits of EIF4F are a 50-kD polopeptied, terned EIF4A and a 220-kD polypeptied EIF4G. EIF4E mediates the binding to the mRNA cap then repress the translation. Due to the increasing expression of c-Myc in the oncogene, EIF4E's activity is enhance. This is a rabbit polyclonal antibody raised against the full-length of human EIF4E. This protein can form a dimer (~50 kDa).
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