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EIF4E Polyclonal antibody

EIF4E Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 11149-1-AP

Synonyms

CBP, CBP,EIF4E, eIF 4E, eIF 4F 25 kDa subunit, EIF4E, EIF4E1, EIF4EL1, EIF4F, mRNA cap binding protein



Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, HEK-293T cells, HepG2 cells, Jurkat cells, K-562 cells
Positive IHC detected inhuman breast cancer tissue, human gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

11149-1-AP targets EIF4E in WB, IHC, IF, FC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EIF4E fusion protein Ag1626
Full Name eukaryotic translation initiation factor 4E
Calculated molecular weight 29 kDa
Observed molecular weight 26-29 kDa, 50 kDa
GenBank accession numberBC012611
Gene symbol EIF4E
Gene ID (NCBI) 1977
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Eukaryotic translation initiation factor 4E(EIF4E) is the rate-limiting part of mRNA 7-methyl guanosine cap-binding component of the translation initiation complex eIF4F. The other subunits of EIF4F are a 50-kD polopeptied, terned EIF4A and a 220-kD polypeptied EIF4G. EIF4E mediates the binding to the mRNA cap then repress the translation. Due to the increasing expression of c-Myc in the oncogene, EIF4E's activity is enhance. This is a rabbit polyclonal antibody raised against the full-length of human EIF4E. This protein can form a dimer (~50 kDa).

Protocols

Product Specific Protocols
WB protocol for EIF4E antibody 11149-1-APDownload protocol
IHC protocol for EIF4E antibody 11149-1-APDownload protocol
IF protocol for EIF4E antibody 11149-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nucleic Acids Res

SUMOylation of the m6A-RNA methyltransferase METTL3 modulates its function.

Authors - Yuzhang Du
humanWB

Mol Ther

RNA m6A methylation regulates the dissemination of cancer cells via modulating expression and membrane localization of β-catenin.

Authors - Jiexin Li
humanWB

Theranostics

KDM6A promotes imatinib resistance through YY1-mediated transcriptional upregulation of TRKA independently of its demethylase activity in chronic myelogenous leukemia.

Authors - Chengwan Zhang
humanWB

Cell Death Dis

Multifunctional viral protein γ34.5 manipulates nucleolar protein NOP53 for optimal viral replication of HSV-1.

Authors - Wen Meng
humanWB

Viruses

The Natural Compound Homoharringtonine Presents Broad Antiviral Activity In Vitro and In Vivo.

Authors - Hui-Jun Dong
humanWB

FEBS Lett

Identification of the receptor tyrosine kinases (RTKs)-oriented functional targets of miR-206 by an antibody-based protein array.

Authors - Yazhou Cui