CBP20 Polyclonal antibody

CBP20 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 11950-1-AP

Synonyms

NCBP2, NCBP-interacting protein 1, NCBP 20 kDa subunit, Cell proliferation-inducing gene 55 protein, CBC2

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Validation Data Gallery

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  WB analysis of human heart using 11950-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 11950-1-AP (CBP20 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 11950-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11950-1-AP (CBP20 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 11950-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11950-1-AP (CBP20 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 11950-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CBP20 antibody (11950-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Tested Applications

• Positive WB detected in: human heart tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 1 publications below

Product Information

11950-1-AP targets CBP20 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CBP20 fusion protein Ag2550
• Full Name: nuclear cap binding protein subunit 2, 20kDa
• Calculated Molecular Weight: 156 aa, 18 kDa
• Observed Molecular Weight: 18 kDa
• GenBank Accession Number: BC001255
• Gene Symbol: NCBP2
• Gene ID (NCBI): 22916
• RRID: AB_2150142
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	iScience; Multi-omics approach reveals posttranscriptionally regulated genes are essential for human pluripotent stem cells.; Authors - Mio Iwasaki
human	WB,IHC	Eur J Med Res; N7-methylguanosin regulators-mediated methylation modification patterns and characterization of the immune microenvironment in lower-grade glioma; Authors - Aierpati Maimaiti