NCBP1/CBP80 Polyclonal antibody

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• KD/KO Validated

NCBP1/CBP80 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 10349-1-AP Copied!

Publications (15)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, IP, CoIP, ELISA, CUT&Tag

Synonyms

NCBP1, 80 kDa nuclear cap-binding protein, CBP80, NCBP, NCBP 80 kDa subunit

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  WB analysis using 10349-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10349-1-AP (NCBP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HEK-293 using 10349-1-AP

  WB result of NCBP1/CBP80 antibody (10349-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-NCBP1/CBP80 transfected HEK-293 cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HeLa using 10349-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10349-1-AP (NCBP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HEK-293 using 10349-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10349-1-AP (NCBP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HeLa using 10349-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10349-1-AP (NCBP1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human brain using 10349-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10349-1-AP (NCBP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HeLa using 10349-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10349-1-AP (NCBP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HEK-293 using 10349-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10349-1-AP (NCBP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of HeLa using 10349-1-AP

  IP result of anti-NCBP1 (IP:10349-1-AP, 4ug; Detection:10349-1-AP 1:500) with HeLa cells lysate 2480ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human lung cancer using 10349-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 10349-1-AP (NCBP1 antibody) at dilution of 1:100 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human kidney using 10349-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 10349-1-AP (NCBP1 antibody) at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human kidney using 10349-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 10349-1-AP (NCBP1 antibody) at dilution of 1:50 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, human brain tissue, Jurkat cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human lung cancer tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

10349-1-AP targets NCBP1/CBP80 in WB, IHC, IF, IP, CoIP, ELISA, CUT&Tag applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, monkey
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag0496

  Product name: Recombinant human CBP80 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 46-350 aa of BC001450

  Sequence: LESNLEGLAGVLEADLPNYKSKILRLLCTVARLLPEKLTIYTTLVGLLNARNYNFGGEFVEAMIRQLKESLKANNYNEAVYLVRFLSDLVNCHVIAAPSMVAMFENFVSVTQEEDVPQVRRDWYVYAFLSSLPWVGKELYEKKDAEMDRIFANTESYLKRRQKTHVPMLQVWTADKPHPQEEYLDCLWAQIQKLKKDRWQERHILRPYLAFDSILCEALQHNLPPFTPPPHTEDSVYPMPRVIFRMFDYTDDPEGPVMPGSHSVERFVIEENLHCIIKSHWKERKTCAAQLVSYPGKNKIPLNYH

• Full Name: nuclear cap binding protein subunit 1, 80kDa
• Calculated Molecular Weight: 92 kDa
• Observed Molecular Weight: 80 kDa
• GenBank Accession Number: BC001450
• Gene Symbol: NCBP1
• Gene ID (NCBI): 4686
• RRID: AB_2150127
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q09161
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Nuclear cap binding protein subunit 1, 80kDa (NCBP1, synonyms: NCBP, CBP80) is a subunit of a cap-binding protein complex (CBC) that consists of a CBP20 subunit, which binds the cap, and a NCBP1 subunit, which ensures high-affinity cap binding. The CBC complex may play a role in pre-mRNA recognition and splicing.

Publications

What published studies show

The NCBP1/CBP80 Polyclonal antibody (Cat# 10349-1-AP) has 15 citations published in journals including Cell, Nature Communications, Molecular Cell, Nucleic Acids Research, Genes & Development, Science Signaling, Neurobiology of Disease, Molecular Neurobiology, Cancer Science, Scientific Reports, European Journal of Medical Research, Journal of Cellular and Molecular Medicine, and Methods in Enzymology. Research fields span RNA methylation and processing, nonsense-mediated mRNA decay, cancer biology, and neurology.

Automatically generated from the 15 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Cell; RNA-Methylation-Dependent RNA Processing Controls the Speed of the Circadian Clock.; Authors - Fustin Jean-Michel JM ; KD Validated
human	WB	Nat Commun; METTL3 acetylation impedes cancer metastasis via fine-tuning its nuclear and cytosolic functions; Authors - Yuanpei Li
human	WB	Mol Cell; Efficiency of the pioneer round of translation affects the cellular site of nonsense-mediated mRNA decay.; Authors - Hanae Sato
mouse	WB,IP	Mol Cell; RNA Pol II preferentially regulates ribosomal protein expression by trapping disassociated subunits; Authors - Yuanjun Li
human	WB	Nucleic Acids Res; SUMOylation of the m6A-RNA methyltransferase METTL3 modulates its function.; Authors - Yuzhang Du
human	WB,IP	Genes Dev; SMG-8 and SMG-9, two novel subunits of the SMG-1 complex, regulate remodeling of the mRNA surveillance complex during nonsense-mediated mRNA decay.; Authors - Yamashita Akio A