|Positive WB detected in||HEK-293 cells, HeLa cells, human brain tissue|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human lung cancer tissue, human kidney tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
10349-1-AP targets NCBP1 in WB, IP, IHC,ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, monkey, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||NCBP1 fusion protein Ag0496|
|Full Name||nuclear cap binding protein subunit 1, 80kDa|
|Calculated molecular weight||92 kDa|
|Observed molecular weight||80 kDa|
|GenBank accession number||BC001450|
|Gene ID (NCBI)||4686|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Nuclear cap binding protein subunit 1, 80kDa (NCBP1, synonyms: NCBP, CBP80) is a subunit of a cap-binding protein complex (CBC) that consists of a CBP20 subunit, which binds the cap, and a NCBP1 subunit, which ensures high-affinity cap binding. The CBC complex may play a role in pre-mRNA recognition and splicing.
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