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NCBP1 Polyclonal antibody

NCBP1 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IHC,ELISA

Conjugate

Unconjugated

Cat no : 10349-1-AP

Synonyms

CBP80, NCBP, NCBP 80 kDa subunit, NCBP1



Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, human brain tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman lung cancer tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

10349-1-AP targets NCBP1 in WB, IP, IHC,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, monkey, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NCBP1 fusion protein Ag0496
Full Name nuclear cap binding protein subunit 1, 80kDa
Calculated molecular weight 92 kDa
Observed molecular weight 80 kDa
GenBank accession numberBC001450
Gene symbol NCBP1
Gene ID (NCBI) 4686
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Nuclear cap binding protein subunit 1, 80kDa (NCBP1, synonyms: NCBP, CBP80) is a subunit of a cap-binding protein complex (CBC) that consists of a CBP20 subunit, which binds the cap, and a NCBP1 subunit, which ensures high-affinity cap binding. The CBC complex may play a role in pre-mRNA recognition and splicing.

Protocols

Product Specific Protocols
WB protocol for NCBP1 antibody 10349-1-APDownload protocol
IHC protocol for NCBP1 antibody 10349-1-APDownload protocol
IP protocol for NCBP1 antibody 10349-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,monkey,mouseIP

Methods Enzymol

Chapter 9. Studying nonsense-mediated mRNA decay in mammalian cells.

Authors - Matsuda Daiki D
humanWB

Cancer Sci

Heat shock protein 90 regulates phosphatidylinositol 3-kinase-related protein kinase family proteins together with the RUVBL1/2 and Tel2-containing co-factor complex.

Authors - Izumi Natsuko N
humanWB

Sci Signal

AAA+ proteins RUVBL1 and RUVBL2 coordinate PIKK activity and function in nonsense-mediated mRNA decay.

Authors - Izumi Natsuko N
humanWB

Nucleic Acids Res

SUMOylation of the m6A-RNA methyltransferase METTL3 modulates its function.

Authors - Yuzhang Du
humanWB,IP

Genes Dev

SMG-8 and SMG-9, two novel subunits of the SMG-1 complex, regulate remodeling of the mRNA surveillance complex during nonsense-mediated mRNA decay.

Authors - Yamashita Akio A
humanWB

Mol Cell

Efficiency of the pioneer round of translation affects the cellular site of nonsense-mediated mRNA decay.

Authors - Hanae Sato