CBX1 Polyclonal antibody

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  WB analysis using 10241-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10241-2-AP (CBX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 10241-2-AP

  WB result of CBX1 antibody (10241-2-AP; 1:2400; incubated at room temperature for 1.5 hours) with sh-Control and sh-CBX1 transfected MCF-7 cells.

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  WB analysis of HEK-293 using 10241-2-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10241-2-AP (CBX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 10241-2-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10241-2-AP (CBX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 10241-2-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 10241-2-AP (CBX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 10241-2-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10241-2-AP (CBX1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 10241-2-AP

  IP result of anti-CBX1 (IP:10241-2-AP, 3ug; Detection:10241-2-AP 1:500) with HEK-293 cells lysate 1480ug.

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  IHC staining of mouse testis using 10241-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10241-2-AP (CBX1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 10241-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10241-2-AP (CBX1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 10241-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10241-2-AP (CBX1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 10241-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10241-2-AP (CBX1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 10241-2-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10241-2-AP (CBX1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 10241-2-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10241-2-AP (CBX1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 10241-2-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10241-2-AP (CBX1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 10241-2-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10241-2-AP (CBX1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 10241-2-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10241-2-AP (CBX1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 10241-2-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10241-2-AP (CBX1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HEK-293 using 10241-2-AP

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using CBX1 antibody (10241-2-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-Phalloidin (green).

• Featured Product
• KD/KO Validated

CBX1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 10241-2-AP

Publications(10)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

HP1Hsbeta, HP1Hs beta, HP1 beta, Heterochromatin protein p25, Chromobox protein homolog 1

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Tested Applications

• Positive WB detected in: A431 cells, MCF-7 cells, HEK-293 cells, human brain tissue, NIH/3T3 cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human stomach cancer tissue, mouse testis tissue, human colon cancer tissue, human lung cancer tissue, human ovary tumor tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 9 publications below
• IHC: See 2 publications below
• IF: See 3 publications below
• IP: See 2 publications below

Product Information

10241-2-AP targets CBX1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CBX1 fusion protein Ag0328
• Full Name: chromobox homolog 1 (HP1 beta homolog Drosophila )
• Calculated Molecular Weight: 27 kDa
• Observed Molecular Weight: 27 kDa
• GenBank Accession Number: BC002609
• Gene Symbol: CBX1
• Gene ID (NCBI): 10951
• RRID: AB_2228586
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P83916
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

One function of heterochromatin is the epigenetic silencing by sequestration of genes into transcriptionally repressed nuclear neighborhoods. Heterochromatin protein 1 (HP1) is a major component of heterochromatin and is a candidate for establishing and mediating gene silencing. This protein is expressed in all adult and embryonic tissues, as a homodimer.

Publications

Species	Application	Title
mouse	WB,IP	J Exp Med; ZNF382 controls mouse neuropathic pain via silencer-based epigenetic inhibition of Cxcl13 in DRG neurons.; Authors - Longfei Ma
mouse	WB	Nat Commun; The histone H3K9 methyltransferase SUV39H links SIRT1 repression to myocardial infarction.; Authors - Guang Yang
human	WB,IHC,IF	J Cell Biol; Induction of alternative lengthening of telomeres-associated PML bodies by p53/p21 requires HP1 proteins.; Authors - Jiang Wei-Qin WQ ; KD Validated
human	WB	J Virol; PIWIL4 Maintains HIV-1 Latency by Enforcing Epigenetically Suppressive Modifications on the 5' Long Terminal Repeat.; Authors - Zhangping He
human	WB,IF	Front Genet; Identification and Validation of Chromobox Family Members as Potential Prognostic Biomarkers and Therapeutic Targets for Human Esophageal Cancer.; Authors - Xuefen Fang
human	WB,IHC,IP	Adv Sci (Weinh); N^6 -Methyladenosine-Modified CBX1 Regulates Nasopharyngeal Carcinoma Progression Through Heterochromatin Formation and STAT1 Activation; Authors - Yin Zhao

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

WEI (Verified Customer) (03-08-2022)

Band at predicted size

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse Heart