CBX4 Polyclonal antibody

CBX4 Polyclonal Antibody for IF, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF,ELISA

Conjugate

Unconjugated

Cat no : 18544-1-AP

Synonyms

CBX4, Chromobox protein homolog 4, E3 SUMO protein ligase CBX4, hPC2, NBP16, PC2, Polycomb 2 homolog



Tested Applications

Positive WB detected inVarious cell, HEK-293 cell, PC-3 cells
Positive IF detected inU2OS cell, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:50-1:400
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 18544-1-AP is CBX4 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CBX4 fusion protein Ag13425
Full Name chromobox homolog 4 (Pc class homolog, Drosophila)
Calculated molecular weight 61 kDa
Observed molecular weight ~75 kDa
GenBank accession numberBC014967
Gene symbol CBX4
Gene ID (NCBI) 8535
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CBX4, also named as E3 SUMO-protein ligase CBX4 or Polycomb 2 homolog, is a 560 amino acid protein, which contains 1 chromo domain. CBX4 facilitates SUMO1 conjugation by UBE2I. CBX4 acting as a transcriptional co-repressor is involved in maintaining the transcriptionally repressive state of genes, modifies chromatin, rendering it heritably changed in its expressibility. CBX4 localizes in the nucleus and is widely expressed in various tissues. The calculated molecular weight of CBX4 is 61 kDa, but the modified CBX4 may be a 70-75 kDa protein.

Protocols

Product Specific Protocols
WB protocol for CBX4 antibody 18544-1-APDownload protocol
IF protocol for CBX4 antibody 18544-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Bioengineered

Long intergenic noncoding RNA00265 promotes proliferation of gastric cancer via the microRNA-144-3p/Chromobox 4 axis.

Authors - Zengxi Yang
  • KD Validated