|Positive WB detected in||Various cell, HEK-293 cell, PC-3 cells|
|Positive IF detected in||U2OS cell, HeLa cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunofluorescence (IF)||IF : 1:50-1:400|
|Sample-dependent, check data in validation data gallery|
The immunogen of 18544-1-AP is CBX4 Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||CBX4 fusion protein Ag13425|
|Full Name||chromobox homolog 4 (Pc class homolog, Drosophila)|
|Calculated molecular weight||61 kDa|
|Observed molecular weight||~75 kDa|
|GenBank accession number||BC014967|
|Gene ID (NCBI)||8535|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
CBX4, also named as E3 SUMO-protein ligase CBX4 or Polycomb 2 homolog, is a 560 amino acid protein, which contains 1 chromo domain. CBX4 facilitates SUMO1 conjugation by UBE2I. CBX4 acting as a transcriptional co-repressor is involved in maintaining the transcriptionally repressive state of genes, modifies chromatin, rendering it heritably changed in its expressibility. CBX4 localizes in the nucleus and is widely expressed in various tissues. The calculated molecular weight of CBX4 is 61 kDa, but the modified CBX4 may be a 70-75 kDa protein.
Front Cell Dev Biol
SALL1 Modulates CBX4 Stability, Nuclear Bodies, and Regulation of Target Genes.
Long intergenic noncoding RNA00265 promotes proliferation of gastric cancer via the microRNA-144-3p/Chromobox 4 axis.
CBX4 promotes anti-tumor immunity by suppressing Pdcd1 expression in T cells