CBX4 Polyclonal antibody

• Featured Product
• KD/KO Validated

CBX4 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA, PLA

Conjugate

Unconjugated

Publications

3

Cat no : 18544-1-AP

Synonyms

Pc2, NBP16, hPc2, EC:2.3.2.-, E3 SUMO-protein ligase CBX4

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Validation Data Gallery

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  WB analysis of HEK-293 cell using 18544-1-AP

  WB result of anti-CBX4 (18544-1-AP) with various cell. (Courtesy of L. Pirone and R. Barrio, CIC bioGUNE).

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  WB analysis of PC-3 using 18544-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 18544-1-AP (CBX4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 18544-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 18544-1-AP (CBX4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 18544-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 18544-1-AP (CBX4 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HeLa using 18544-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CBX4 antibody (18544-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

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Tested Applications

• Positive WB detected in: Various cell, HEK-293 cell, PC-3 cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 1 publications below

Product Information

18544-1-AP targets CBX4 in WB, IF/ICC, ELISA, PLA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CBX4 fusion protein Ag13425
• Full Name: chromobox homolog 4 (Pc class homolog, Drosophila)
• Calculated Molecular Weight: 61 kDa
• Observed Molecular Weight: ~75 kDa
• GenBank Accession Number: BC014967
• Gene Symbol: CBX4
• Gene ID (NCBI): 8535
• RRID: AB_2878552
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CBX4, also named as E3 SUMO-protein ligase CBX4 or Polycomb 2 homolog, is a 560 amino acid protein, which contains 1 chromo domain. CBX4 facilitates SUMO1 conjugation by UBE2I. CBX4 acting as a transcriptional co-repressor is involved in maintaining the transcriptionally repressive state of genes, modifies chromatin, rendering it heritably changed in its expressibility. CBX4 localizes in the nucleus and is widely expressed in various tissues. The calculated molecular weight of CBX4 is 61 kDa, but the modified CBX4 may be a 70-75 kDa protein.

Publications

Species	Application	Title
human	WB,IF,PLA	Front Cell Dev Biol; SALL1 Modulates CBX4 Stability, Nuclear Bodies, and Regulation of Target Genes.; Authors - Immacolata Giordano
human	WB	Bioengineered; Long intergenic noncoding RNA00265 promotes proliferation of gastric cancer via the microRNA-144-3p/Chromobox 4 axis.; Authors - Zengxi Yang ; KD Validated
human	WB	Mol Oncol; CBX4 promotes anti-tumor immunity by suppressing Pdcd1 expression in T cells; Authors - Liwei Ren