CBX6 Polyclonal antibody

CBX6 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 23426-1-AP

Synonyms

CBX6, chromobox homolog 6, Chromobox protein homolog 6

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Validation Data Gallery

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  WB analysis of HepG2 using 23426-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 23426-1-AP (CBX6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 23426-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23426-1-AP (CBX6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 23426-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 23426-1-AP (CBX6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 23426-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 23426-1-AP (CBX6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human skin using 23426-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin slide using 23426-1-AP (CBX6 Antibody) at dilution of 1:50.

• 

  IHC staining of human skin using 23426-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin slide using 23426-1-AP (CBX6 Antibody) at dilution of 1:50.

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Tested Applications

• Positive WB detected in: HepG2 cells, HEK-293 cells, HeLa cells
• Positive IHC detected in: human skin tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

The immunogen of 23426-1-AP is CBX6 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CBX6 fusion protein Ag20014
• Full Name: chromobox homolog 6
• Calculated Molecular Weight: 412 aa, 44 kDa
• Observed Molecular Weight: 55-60 kDa
• GenBank Accession Number: BC064900
• Gene Symbol: CBX6
• Gene ID (NCBI): 23466
• RRID: AB_2737364
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The Chromobox domain (Cbx) gene family, consisting of Polycomb and Heterochromatin Protein 1 genes, involves in transcriptional repression, cell cycle regulation and chromatin remodeling [PMID: 17486619]. Cbx6, other named neuronal pentraxin receptor (NPR), first described as a putative integral membrane pentraxin that interacts with neuronal pentraxin 1 and 2, has also been classified as a Pc protein [PMID: 14647293]. NPR was determined to be a member of a distinct pentraxin protein subfamily, and found to form heteropentamer swithNP1 andNP2, followed by release from cell membranes [PMID: 10748068].

Publications

Species	Application	Title
human	WB	Cell; Targeting Epigenetic Crosstalk as a Therapeutic Strategy for EZH2-Aberrant Solid Tumors.; Authors - Xun Huang