CCAR2 Monoclonal antibody

• Featured Product
• KD/KO Validated

CCAR2 Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, pig

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

2D10F8

Cat no : 66497-1-Ig

Synonyms

CCAR2, cell cycle and apoptosis regulator 2, DBC 1, DBC.1, DBC1, KIAA1967, p30 DBC, Protein KIAA1967

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Validation Data Gallery

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  WB analysis using 66497-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66497-1-Ig (CCAR2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 66497-1-Ig

  WB result of CCAR2 antibody (66497-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CCAR2 transfected HEK-293 cells.

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  WB analysis of pig brain using 66497-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 66497-1-Ig (CCAR2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IHC staining of human cervical cancer using 66497-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66497-1-Ig (CCAR2 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 66497-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66497-1-Ig (CCAR2 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 66497-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66497-1-Ig (CCAR2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 66497-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66497-1-Ig (CCAR2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 66497-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66497-1-Ig (CCAR2 antibody) at dilution of 1:32000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 66497-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66497-1-Ig (CCAR2 antibody) at dilution of 1:32000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 66497-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66497-1-Ig (CCAR2 antibody) at dilution of 1:500 (under 10x lens).

• 

  IHC staining of human colon using 66497-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66497-1-Ig (CCAR2 antibody) at dilution of 1:500 (under 40x lens).

• 

  IHC staining of human lung cancer using 66497-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66497-1-Ig (CCAR2 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 66497-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66497-1-Ig (CCAR2 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 66497-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 66497-1-Ig (CCAR2 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  FC experiment of U2OS using 66497-1-Ig

  1X10^6 U2OS cells were intracellularly stained with 0.2 ug Anti-Human CCAR2 (66497-1-Ig, Clone:2D10F8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Mouse IgG2a Isotype Control (66360-2-Ig, Clone: K11A1B2A2) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

• Positive WB detected in: HeLa cells, pig brain tissue, HEK-293 cells, MCF-7 cells, Jurkat cells
• Positive IHC detected in: human cervical cancer tissue, human colon tissue, human lung cancer tissue, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells
• Positive FC detected in: U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Product Information

66497-1-Ig targets CCAR2 in WB, IHC, IF, FC, ELISA applications and shows reactivity with Human, pig samples.

• Tested Reactivity: Human, pig
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: CCAR2 fusion protein Ag18374
• Full Name: KIAA1967
• Calculated molecular weight: 923 aa, 103 kDa
• Observed molecular weight: 130 kDa
• GenBank accession number: BC018269
• Gene symbol: KIAA1967
• Gene ID (NCBI): 57805
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cell cycle and apoptosis regulator protein 2 (CCAR2), previously known as DBC1/KIAA1967, regulates diverse cellular functions including transcription, mRNA splicing, metabolism, and circadian cycle. CCAR2 is a critical regulator of cell death or survival following cellular stresses. CCAR2 is a widely expressed protein involved in the regulation of a variety of transcriptional complexes. High expression of CCAR2 correlates with poor outcomes in many human tumor types such as squamous cell carcinoma (SCC).