CCDC102B Polyclonal antibody

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  IP experiment of MOLT-4 using 31675-1-AP

  IP result of anti-CCDC102B (IP:31675-1-AP, 4ug; Detection:31675-1-AP 1:800) with MOLT-4 cells lysate 1800 ug.

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  IP experiment of SH-SY5Y using 31675-1-AP

  IP result of anti-CCDC102B (IP:31675-1-AP, 4ug; Detection:31675-1-AP 1:800) with SH-SY5Y cells lysate 1240 ug.

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  IF Staining of A431 using 31675-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using CCDC102B antibody (31675-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

CCDC102B Polyclonal Antibody for IF/ICC, IP, ELISA

Cat No. 31675-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, IP, ELISA

Synonyms

ACY1L, C18orf14

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Tested Applications

• Positive IP detected in: SH-SY5Y cells, MOLT-4 cells
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31675-1-AP targets CCDC102B in IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag36379

  Product name: Recombinant human CCDC102B protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-240 aa of BC126448

  Sequence: MKPNLDGVDLFNNGGSGNGETKTGLRLKAINLPLENEVTEISALQVHLDEFQKILWKEREMRTALEKEIERLESALSLWKWKYEELKESKPKNVKEFDILLGQHNDEMQELSGNIKEESKSQNSKDRVICELRAELERLQAENTSEWDKREILEREKQGLERENRRLKIQVKEMEELLDKKNRLSANSQSPDFKMSQIDLQEKNQELLNLQHAYYKLNRQYQANIAELTHANNRVDQNEA

• Full Name: coiled-coil domain containing 102B
• Observed Molecular Weight: 60~68 kDa
• GenBank Accession Number: BC126448
• Gene Symbol: CCDC102B
• Gene ID (NCBI): 79839
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q68D86
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Coiled coil domain containing 102B (CCDC102B) is a new centrosome-linker protein that is required for maintaining centrosome cohesion. Human CCDC102B is an uncharacterized protein containing 513 amino acids (aa); it is predicted to contain one short coiled-coil domain on its N-terminus and two long coiled-coil domains in the middle region and C-terminus, respectively (PMID: 35957886). At the onset of mitosis, along with other centrosome components, CCDC102B is phosphorylated by Nek2A and is disassociated from the centrosome to allow for the centrosome separation (PMID: 30404835).