CCDC109B Polyclonal antibody

• 

  WB analysis of MCF-7 using 20387-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 20387-1-AP (CCDC109B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

CCDC109B Polyclonal Antibody for WB, ELISA

Cat No. 20387-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, ELISA

Synonyms

CCDC109B, MCUb

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Tested Applications

• Positive WB detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below

Product Information

20387-1-AP targets CCDC109B in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag14225

  Product name: Recombinant human CCDC109B protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-230 aa of BC002633

  Sequence: MLQRGLWPWRTRLLPTPGTWRPARPWPLPPPPQVLRVKLCGNVKYYQSHHYSTVVPPDEITVIYRHGLPLVTLTLPSRKERCQFVVKPMLSTVGSFLQDLQNEDKGIKTAAIFTADGNMISASTLMDILLMNDFKLVINKIAYDVQCPKREKPSNEHTAEMEHMKSLVHRLFTILHLEESQKKREHHLLEKIDHLKEQLQPLEQVKAGIEAHSEAKTSGLLWAGLALLSI

• Full Name: coiled-coil domain containing 109B
• Calculated Molecular Weight: 336 aa, 39 kDa
• Observed Molecular Weight: 40-45 kDa
• GenBank Accession Number: BC002633
• Gene Symbol: CCDC109B
• Gene ID (NCBI): 55013
• RRID: AB_2878681
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9NWR8
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Neurobiol Aging; Modulation of OSCP mitigates mitochondrial and synaptic deficits in a mouse model of Alzheimer's pathology.; Authors - Esha Gauba
mouse	WB	Immunol Lett; Modulation of Oxidized Low-density Lipoprotein-Affected Macrophage Efferocytosis by Mitochondrial Calcium Uniporter in a Murine Model; Authors - Na Lu
mouse	WB	Biol Trace Elem Res; The ROS Mediates MCUb in Mitochondria-Regulated Apoptosis of TM4 Cells Induced by Titanium Dioxide Nanoparticles; Authors - Chenhao Sun