CCDC115 Polyclonal antibody

• Home
• Products
• Primary Antibodies
• CCDC115 Polyclonal antibody (20636-1-AP)

CCDC115 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 20636-1-AP Copied!

Publications (1)

Download datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

ccp1, Coiled-coil domain-containing protein 115

• 

  WB analysis using 20636-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20636-1-AP (CCDC115 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse brain using 20636-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20636-1-AP (CCDC115 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse brain using 20636-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20636-1-AP (CCDC115 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human ovary cancer using 20636-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 20636-1-AP (CCDC115 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human ovary cancer using 20636-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 20636-1-AP (CCDC115 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human ovary cancer using 20636-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 20636-1-AP (CCDC115 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human placenta using 20636-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 20636-1-AP (CCDC115 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of rat brain using 20636-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 20636-1-AP (CCDC115 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of rat lung using 20636-1-AP

  Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 20636-1-AP (CCDC115 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Able AI

What's the best dilution for CCDC115 in ?

Chat with Able

---

Tested Applications

• Positive WB detected in: A549 cells, mouse brain tissue, MOLT-4 cells, Y79 cells
• Positive IHC detected in: human ovary cancer tissue, rat brain tissue, rat lung tissue, human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

20636-1-AP targets CCDC115 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag14677

  Product name: Recombinant human CCDC115 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-180 aa of BC006429

  Sequence: MAALDLRAELDSLVLQLLGDLEELEGKRTVLNARVEEGWLSLAKARYAMGAKSVGPLQYASHMEPQVCLHASEAQEGLQKFKVVRAGVHAPEEVGPREAGLRRRKGPTKTPEPESSEAPQDPLNWFGILVPHSLRQAQASFRDGLQLAADIASLQNRIDWGRSQLRGLQEKLKQLEPGAA

• Full Name: coiled-coil domain containing 115
• Calculated Molecular Weight: 180 aa, 20 kDa
• Observed Molecular Weight: 22-25 kDa
• GenBank Accession Number: BC006429
• Gene Symbol: CCDC115
• Gene ID (NCBI): 84317
• RRID: AB_10733755
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96NT0
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CCDC115 (Coiled-Coil Domain-Containing Protein 115), also known as CCP1 or CDG2O,is a deduced 180-amino acid protein containing two coiled-coil domains, a putative leucine zipper, and an N-myristoylation site. CCDC115 serves as an accessory component of the vacuolar (V)-ATPase proton pump, playing essential roles in intracellular iron homeostasis and endolysosomal acidification.

Publications

What published studies show

The antibody Anti-CCDC115 (Cat# 20636-1-AP) has 1 total citation. It appears in the journal Heliyon (Impact Factor: 4), published in 2023. The associated research field is liver cancer, specifically identifying CCDC115 as an adverse prognostic biomarker through bioinformatics and experimental analyses. The application used was Western Blot (WB) in human samples.

Automatically generated from the 1 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Heliyon; Identification of CCDC115 as an adverse prognostic biomarker in liver cancer based on bioinformatics and experimental analyses; Authors - Chang Su