CCDC120 Polyclonal antibody

• Featured Product
• KD/KO Validated

CCDC120 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 22041-1-AP

Synonyms

CCDC120, JM11

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Validation Data Gallery

• 

  WB analysis of Jurkat using 22041-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 22041-1-AP (CCDC120 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 22041-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 22041-1-AP (CCDC120 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon using 22041-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 22041-1-AP (CCDC120 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon using 22041-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 22041-1-AP (CCDC120 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human placenta using 22041-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 22041-1-AP (CCDC120 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human placenta using 22041-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 22041-1-AP (CCDC120 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: Jurkat cells, mouse liver tissue
• Positive IHC detected in: human colon tissue, human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

The immunogen of 22041-1-AP is CCDC120 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CCDC120 fusion protein Ag14319
• Full Name: coiled-coil domain containing 120
• Calculated Molecular Weight: 630 aa, 68 kDa
• Observed Molecular Weight: 67-70 kDa
• GenBank Accession Number: BC008769
• Gene Symbol: CCDC120
• Gene ID (NCBI): 90060
• RRID: AB_11182175
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CCDC120, also named as JM11, is required for neurite growth. In differentiating neuroblastoma cells, CCDC120 colocalizes with CYTH2 in both neurite shaft and growth cone areas. CCDC120 has some isoforms with MW 67-70 kDa.

Publications

Species	Application	Title
human	IP	Nat Commun; Hierarchical assembly of centriole subdistal appendages via centrosome binding proteins CCDC120 and CCDC68.; Authors - Ning Huang
mouse	WB,IF	J Biol Chem; Arf6 guanine-nucleotide exchange factor cytohesin-2 binds to CCDC120 and is transported along neurites to mediate neurite growth.; Authors - Tomohiro Torii ; KD Validated