CCDC124 Polyclonal antibody

CCDC124 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 27106-1-AP

Synonyms

CCDC124

---

Validation Data Gallery

• 

  WB analysis of mouse brain using 27106-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 27106-1-AP (CCDC124 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 27106-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 27106-1-AP (CCDC124 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 27106-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 27106-1-AP (CCDC124 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HeLa using 27106-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 27106-1-AP (CCDC124 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 27106-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 27106-1-AP (CCDC124 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive WB detected in: mouse brain tissue, human placenta tissue, mouse liver tissue
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 1 publications below

Product Information

The immunogen of 27106-1-AP is CCDC124 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CCDC124 fusion protein Ag25930
• Full Name: coiled-coil domain containing 124
• Observed Molecular Weight: 30-32 kDa
• GenBank Accession Number: BC013949
• Gene Symbol: CCDC124
• Gene ID (NCBI): 115098
• RRID: AB_2880758
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CCDC124 is a novel centrosomal and midbody protein that is highly conserved among species. It is ubiquitously expressed with high expression in brain, placenta, liver, spleen, and prostate. At telophase, CCDC124 dissociates from centrosomes and relocalizes to the midzone, subsequently accumulating at the midbody at cytokinesis. It interacts with the Ras-guanine nucleotide exchange factor 1B (RasGEF1B) and is involved in cytokinesis.

Publications

Species	Application	Title
human	WB,IF	Protein J; Live Cell Protein Imaging of Tandem Complemented-GFP11-Tagged Coiled-Coil Domain-Containing Protein-124 Identifies this Factor in G3BP1-Induced Stress-Granules; Authors - Kübra Hacibeyoğlu