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Tested Applications
| Positive IP detected in | mouse testis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
27739-1-AP targets CCDC147 in IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag26943 Product name: Recombinant human CCDC147 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-242 aa of BC109126 Sequence: MAEEKGGKQVLEESAFEEMERDFQGVLHELSGDKSLEKFRIEYERLHAVMKKSYDNEKRLMAKCRELNAEIVVNSAKVATALKLSQDDQTTIASLKKEIEKAWKMVDSAYDKEQKAKETILALKEEIVNLTKLVEQGSGLSMDQHSNIRDLLRFKEEVTKERDQLLSEVVKLRESLAQTTEQQQETERSKEEAEHAISQFQQEIQQRQNEASREFRKKEKLEKELKQIQADMDSRQTEIKAL Predict reactive species |
| Full Name | coiled-coil domain containing 147 |
| Calculated Molecular Weight | 872 aa, 103 kDa |
| Observed Molecular Weight | 103 kDa |
| GenBank Accession Number | BC109126 |
| Gene Symbol | CCDC147 |
| Gene ID (NCBI) | 159686 |
| RRID | AB_3742252 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q5T655 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CCDC147 has an essential role in the assembly and organization of the sperm flagellar axoneme (PMID: 32791035).
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for CCDC147 antibody 27739-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
