CCDC22 Polyclonal antibody

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• CCDC22 Polyclonal antibody (16636-1-AP)

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• KD/KO Validated

CCDC22 Polyclonal Antibody for WB, IP, ELISA

Cat No. 16636-1-AP Copied!

Publications (22)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, CoIP, ELISA

Synonyms

coiled coil domain containing 22, Coiled coil domain containing protein 22, Coiled-coil domain-containing protein 22, CXorf37, JM1

• 

  WB analysis using 16636-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16636-1-AP (CCDC22 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HepG2 using 16636-1-AP

  WB result of CCDC22 antibody (16636-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CCDC22 transfected HepG2 cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of rat colon using 16636-1-AP

  rat colon tissue were subjected to SDS PAGE followed by western blot with 16636-1-AP (CCDC22 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human brain using 16636-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 16636-1-AP (CCDC22 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse colon using 16636-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 16636-1-AP (CCDC22 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse lung using 16636-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 16636-1-AP (CCDC22 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of mouse lung using 16636-1-AP

  IP result of anti-CCDC22 (IP:16636-1-AP, 4ug; Detection:16636-1-AP 1:600) with mouse lung tissue lysate 3600ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HEK-293 cells, HepG2 cells, human brain tissue, mouse colon tissue, mouse lung tissue, rat colon tissue, HeLa cells, Jurkat cells
• Positive IP detected in: mouse lung tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16636-1-AP targets CCDC22 in WB, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag9980

  Product name: Recombinant human CCDC22 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 283-628 aa of BC000972

  Sequence: AKTGAPKGSRFTHSEKFTFHLEPQAQATQVSDVPATSRRPEQVTWAAQEQELESLREQLEGVNRSIEEVEADMKTLGVSFVQAESECRHSKLSTAEREQALRLKSRAVELLPDGTANLAKLQLVVENSAQRVIHLAGQWEKHRVPLLAEYRHLRKLQDCRELESSRRLAEIQELHQSVRAAAEEARRKEEVYKQLMSELETLPRDVSRLAYTQRILEIVGNIRKQKEEITKILSDTKELQKEINSLSGKLDRTFAVTDELVFKDAKKDDAVRKAYKYLAALHENCSQLIQTIEDTGTIMREVRDLEEQIETELGKKTLSNLEKIREDYRALRQENAGLLGRVREA

• Full Name: coiled-coil domain containing 22
• Calculated Molecular Weight: 71 kDa
• Observed Molecular Weight: 71 kDa
• GenBank Accession Number: BC000972
• Gene Symbol: CCDC22
• Gene ID (NCBI): 28952
• RRID: AB_2072065
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O60826
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The CCDC22 Polyclonal antibody (Cat# 16636-1-AP) has 22 citations. These appear in journals including Cell, Nature Cell Biology, Nature Communications, Science Immunology, Molecular Metabolism, Journal of Cell Biology, Molecular Cancer Research, mBio, Journal of Molecular Biology, Structure, iScience, Disease Models & Mechanisms, American Journal of Physiology, Molecular Biology Reports, Molecular Biology of the Cell, Molecular Biosystems, bioRxiv, and Molecular Metabolism. Research fields span endosomal/retromer membrane trafficking, viral entry (SARS-CoV-2), copper homeostasis, Notch signaling, cholesterol metabolism, and cancer.

Automatically generated from the 22 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Cell; Structure of the endosomal Commander complex linked to Ritscher-Schinzel syndrome; Authors - Michael D Healy
human	WB	Cell; Identification of Required Host Factors for SARS-CoV-2 Infection in Human Cells.; Authors - Zharko Daniloski
human	WB	Nat Cell Biol; Retriever is a multiprotein complex for retromer-independent endosomal cargo recycling.; Authors - Kerrie E McNally
human	WB	Nat Commun; Mechanism and regulation of cargo entry into the Commander endosomal recycling pathway; Authors - Rebeka Butkovič
human	WB	Nat Commun; A genome-wide CRISPR screen identifies host factors that regulate SARS-CoV-2 entry.; Authors - Yunkai Zhu
human	WB	Nat Commun; Identification of a RAB32-LRMDA-Commander membrane trafficking complex reveals the molecular mechanism of human oculocutaneous albinism type 7.; Authors - Rebeka Butkovič ; KO Validated