CCDC22 Recombinant monoclonal antibody

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  WB analysis using 83992-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83992-1-RR (CCDC22 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 83992-1-RR

  WB result of CCDC22 antibody (83992-1-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CCDC22 transfected HepG2 cells.

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  Affinity and Kinetic Characterization of 83992-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83992-1-RR against Human CCDC22 were performed. The affinity constant is 2.71 nM.

• Featured Product
• KD/KO Validated

CCDC22 Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 83992-1-RR

Clone No.241146A5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

JM1, CXorf37, Coiled-coil domain-containing protein 22, Coiled coil domain containing protein 22, coiled coil domain containing 22

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, HEK-293 cells, Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83992-1-RR targets CCDC22 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag9980

  Product name: Recombinant human CCDC22 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 283-628 aa of BC000972

  Sequence: AKTGAPKGSRFTHSEKFTFHLEPQAQATQVSDVPATSRRPEQVTWAAQEQELESLREQLEGVNRSIEEVEADMKTLGVSFVQAESECRHSKLSTAEREQALRLKSRAVELLPDGTANLAKLQLVVENSAQRVIHLAGQWEKHRVPLLAEYRHLRKLQDCRELESSRRLAEIQELHQSVRAAAEEARRKEEVYKQLMSELETLPRDVSRLAYTQRILEIVGNIRKQKEEITKILSDTKELQKEINSLSGKLDRTFAVTDELVFKDAKKDDAVRKAYKYLAALHENCSQLIQTIEDTGTIMREVRDLEEQIETELGKKTLSNLEKIREDYRALRQENAGLLGRVREA

• Full Name: coiled-coil domain containing 22
• Calculated Molecular Weight: 71 kDa
• Observed Molecular Weight: 71 kDa
• GenBank Accession Number: BC000972
• Gene Symbol: CCDC22
• Gene ID (NCBI): 28952
• RRID: AB_3671567
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O60826
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CCDC22 is a ubiquitously expressed coiled-coil domain protein. CCDC22 functions in the regulation of NF-kB (nuclear factor kappa-light-chain-enhancer of activated B cells) by interacting with COMMD (copper metabolism Murr1 domain-containing) proteins. ]