CCDC27 Polyclonal antibody

CCDC27 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 23769-1-AP

Synonyms

CCDC27

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Validation Data Gallery

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  WB analysis of human testis using 23769-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 23769-1-AP (CCDC27 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 23769-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 23769-1-AP (CCDC27 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 23769-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 23769-1-AP (CCDC27 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: human testis tissue
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 23769-1-AP is CCDC27 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CCDC27 fusion protein Ag20676
• Full Name: coiled-coil domain containing 27
• Calculated Molecular Weight: 656 aa, 75 kDa
• Observed Molecular Weight: 75 kDa
• GenBank Accession Number: BC112108
• Gene Symbol: CCDC27
• Gene ID (NCBI): 148870
• RRID: AB_2879319
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CCDC27 also named as coiled coil domain containing 27 is a 656 amino acids protein, which contains a coiled-coil domain. CCDC27 expression is predominantly in male testis and lowly in other tissues. CCDC27 is a candidate gene for low bone mineral density. The function of CCDC27 is still non-known and need to be further studied.