CCDC45 Monoclonal antibody

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  WB analysis using 67755-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67755-1-Ig (CCDC45 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of U2OS using 67755-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using CCDC45 antibody (67755-1-Ig, Clone: 1A12A7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

CCDC45 Monoclonal Antibody for WB, IF/ICC, ELISA

Cat No. 67755-1-Ig

Clone No.1A12A7

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Synonyms

CCDC45, DKFZp667E1824

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Tested Applications

• Positive WB detected in: MOLT-4 cells, Jurkat cells, K-562 cells, HL-60 cells
• Positive IF/ICC detected in: U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67755-1-Ig targets CCDC45 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag27836

  Product name: Recombinant human CCDC45 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 86-349 aa of BC009518

  Sequence: GENIVKGDKESIKNLLEIFDGLLEYLTERISETSHEKSETEQYFKESDRGERLEEPESTKESKSSWKRVSFGRCSLSSEMLGPSWDGDEAESTGEIIRLGDTAHTFSLRSNGAQCPNEMLSKKALASPSSKSHEDMLYPPSVLSKSRTSFVEDTETLSVSGIPNARKLGEPIRAAIPLHPPYHPSEPRAPCPIGKEYLHSSHCSPAVNSTGEHTEFSGDLDDGLFLISKLPKGSKWEVYPAQVQGPRTRKPPKGKRNENRATAS

• Full Name: coiled-coil domain containing 45
• Calculated Molecular Weight: 821 aa, 95 kDa
• Observed Molecular Weight: 95-100 kDa
• GenBank Accession Number: BC009518
• Gene Symbol: CCDC45
• Gene ID (NCBI): 90799
• RRID: AB_2918523
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q96GE4
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.