HAUS1 Polyclonal antibody

HAUS1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 11094-2-AP

Synonyms

CCDC5, Enhancer of invasion cluster, HAUS1, HEI C, HEIC, HsT1461

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Validation Data Gallery

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  WB analysis of human skeletal muscle using 11094-2-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11094-2-AP (HAUS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11094-2-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11094-2-AP (HAUS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11094-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11094-2-AP (HAUS1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 11094-2-AP

  IP result of anti-HAUS1 (IP:11094-2-AP, 4ug; Detection:11094-2-AP 1:500) with HeLa cells lysate 1200ug.

• 

  IHC staining of human pancreas cancer using 11094-2-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11094-2-AP (HAUS1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 11094-2-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11094-2-AP (HAUS1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: human skeletal muscle tissue, HL-60 cells, HeLa cells, MOLT-4 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

11094-2-AP targets HAUS1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HAUS1 fusion protein Ag1569
• Full Name: coiled-coil domain containing 5 (spindle associated)
• Calculated Molecular Weight: 32 kDa
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: BC014003
• Gene Symbol: HAUS1
• Gene ID (NCBI): 115106
• RRID: AB_614986
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HAUS1 is a subunit of the human augmin complex that is involved in microtubule generation and mitotic spindle formation (PMID:27036161). In addition, HAUS1 may play a major role in centrosome integrity and completion of cytokinesis. The depletion of HAUS1 in cells decreases the G2/M cell compartment and induces apoptosis. High HAUS1 expression was associated with several tumour-infiltrating immune cells such as Th2 cells, macrophages, and activated dendritic cells (PMID:35865089). 11094-2-AP can detect the 32 kDa band, which is consistent with the calculated MW.

Publications

Species	Application	Title
human	WB	J Cell Biol; Reconstitution and mechanistic dissection of the human microtubule branching machinery.; Authors - Yaqian Zhang
mouse	WB	Nucleic Acids Res; An RNAi screen of RNA helicases identifies eIF4A3 as a regulator of embryonic stem cell identity; Authors - Dan Li