CCDC50 Polyclonal antibody

• Featured Product
• KD/KO Validated

CCDC50 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 21082-1-AP

Synonyms

C3orf6, CCDC50, DFNA44, Protein Ymer, YMER

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Validation Data Gallery

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  WB analysis using 21082-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21082-1-AP (CCDC50 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 21082-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 21082-1-AP (CCDC50 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 21082-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 21082-1-AP (CCDC50 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 21082-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21082-1-AP (CCDC50 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 21082-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21082-1-AP (CCDC50 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 21082-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 21082-1-AP (CCDC50 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 21082-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 21082-1-AP (CCDC50 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: K-562 cells, L02 cells, mouse lung tissue, mouse skeletal muscle tissue, rat skeletal muscle tissue
• Positive IHC detected in: human kidney tissue, human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 3 publications below

Product Information

21082-1-AP targets CCDC50 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CCDC50 fusion protein Ag15293
• Full Name: coiled-coil domain containing 50
• Calculated Molecular Weight: 482 aa, 56 kDa
• Observed Molecular Weight: 36-38 kDa
• GenBank Accession Number: BC065004
• Gene Symbol: CCDC50
• Gene ID (NCBI): 152137
• RRID: AB_10695770
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CCDC50 encodes Ymer, an effector of epidermal growth factor (EGF)-mediated cell signaling that is ubiquitously expressed in different organs and has been suggested to inhibit down-regulation of the EGF receptor. CCDC50 act as a multifunctional regulator in NF-κB and Fas signaling pathways. In chronic lymphocytic leukaemia (CLL) and mantle cell lymphoma (MCL), CCDC50 is required for the survival of MCL and CLL cancer cells. Recently, CCDC50 was shown to promote hepatocellular carcinoma through the Ras/Foxo4 signaling pathway. (PMID: 34453126, PMID: 32612200, PMID: 17503326)

Publications

Species	Application	Title
human,mouse	WB,IHC,IF	EMBO Rep; CCDC50 promotes tumor growth through regulation of lysosome homeostasis; Authors - Penghui Jia ; KD Validated
human,mouse	WB,IF	EMBO Rep; CCDC50 suppresses NLRP3 inflammasome activity by mediating autophagic degradation of NLRP3; Authors - Yuxin Lin ; KO Validated
human	WB,IF	Autophagy; CCDC50 mediates the clearance of protein aggregates to prevent cellular proteotoxicity; Authors - Yu Ye