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CCDC50 Monoclonal antibody, PBS Only (Detector) (68369-1-PBS)

CCDC50 Monoclonal antibody, PBS Only (Detector)

Name: CCDC50 Monoclonal antibody, PBS Only (Detector), 100ug
Brand: Proteintech
SKU: 68369-1-PBS-100UG

CCDC50 Monoclonal Antibody for WB, FC (Intra), Cytometric bead array, Indirect ELISA

Cat No. 68369-1-PBS

Clone No. 2F12G7

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Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), Cytometric bead array, Indirect ELISA

Synonyms

C3orf6, Coiled-coil domain-containing protein 50, DFNA44, Protein Ymer, YMER

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

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Filter: AllWBFC (Intra)Cytometric bead array sample testCytometric bead array

WB analysis using 68369-1-Ig (same clone as 68369-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 68369-1-Ig (CCDC50 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 68369-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
×
Various lysates were subjected to SDS PAGE followed by western blot with 68369-1-Ig (CCDC50 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 68369-1-PBS in a different storage buffer formulation.
FC experiment of A549 using 68369-1-Ig (same clone as 68369-1-PBS)
1x10^6 A549 cells were intracellularly stained with 0.4 ug CCDC50 Monoclonal antibody (68369-1-Ig, Clone:2F12G7) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 68369-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
×
1x10^6 A549 cells were intracellularly stained with 0.4 ug CCDC50 Monoclonal antibody (68369-1-Ig, Clone:2F12G7) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 68369-1-PBS in a different storage buffer formulation.
Cytometric bead array sample test of MP51849-1
Cytometric bead array sample test of MP51849-1, CCDC50 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68369-2-PBS. Detection antibody: 68369-1-PBS.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
Cytometric bead array standard curve of MP51849-1
Cytometric bead array standard curve of MP51849-1, CCDC50 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68369-2-PBS. Detection antibody: 68369-1-PBS. Standard:Ag15889. Range: 0.781-100 ng/mL.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information

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Product Information

68369-1-PBS targets CCDC50 as part of a matched antibody pair:

MP51849-1: 68369-2-PBS capture and 68369-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity	human, mouse, rat
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Ag15889 Product name: Recombinant human CCDC50 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-306 aa of BC065004 Sequence: MAEVSIDQSKLPGVKEVCRDFAVLEDHTLAHSLQEQEIEHHLASNVQRNRLVQHDLQVAKQLQEEDLKAQAQLQKRYKDLEQQDCEIAQEIQEKLAIEAERRRIQEKKDEDIARLLQEKELQEEKKRKKHFPEFPATRAYADSYYYEDGGMKPRVMKEAVSTPSRMAHRDQEWYDAEIARKLQEEELLATQVDMRAAQVAQDEEIARLLMAEEKKAYKKAKEREKSSLDKRKQDPEWKPKTAKAANSKSKESDEPHHSKNERPARPPPPIMTDGEDADYTHFTNQQSSTRHFSKSESSHKGFHYKH Predict reactive species
Full Name	coiled-coil domain containing 50
Calculated Molecular Weight	482 aa, 56 kDa
Observed Molecular Weight	36-38 kDa
GenBank Accession Number	BC065004
Gene Symbol	CCDC50
Gene ID (NCBI)	152137
RRID	AB_3085089
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein G purification
UNIPROT ID	Q8IVM0
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

CCDC50 encodes Ymer, an effector of epidermal growth factor (EGF)-mediated cell signaling that is ubiquitously expressed in different organs and has been suggested to inhibit down-regulation of the EGF receptor. CCDC50 act as a multifunctional regulator in NF-κB and Fas signaling pathways. In chronic lymphocytic leukaemia (CLL) and mantle cell lymphoma (MCL), CCDC50 is required for the survival of MCL and CLL cancer cells. Recently, CCDC50 was shown to promote hepatocellular carcinoma through the Ras/Foxo4 signaling pathway. (PMID: 34453126, PMID: 32612200, PMID: 17503326)