CCDC55 Polyclonal antibody

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CCDC55 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 21360-1-AP Copied!

Publications (1)

Download datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Synonyms

CCDC55, HSPC095, NSRP1, NSRP70

• 

  WB analysis of COLO 320 using 21360-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 21360-1-AP (CCDC55 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human brain using 21360-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 21360-1-AP (CCDC55 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human brain using 21360-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 21360-1-AP (CCDC55 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: COLO 320 cells
• Positive IHC detected in: human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

21360-1-AP targets CCDC55 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag15811

  Product name: Recombinant human CCDC55 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 216-558 aa of BC040118

  Sequence: GFSNEVSSKNRIPQEKCILQTDVKVEENPDADSDFDAKSSADDEIEETRVNCRREKVIETPENDFKHHRSQNHSRSPSEERGHSTRHHTKGSRTSRGHEKREDQHQQKQSRDQENHYTDRDYRKERDSHRHREASHRDSHWKRHEQEDKPRARDQRERSDRVWKREKDREKYSQREQERDRQQNDQNRPSEKGEKEEKSKAKEEHMKVRKERYENNDKYRDREKREVGVQSSERNQDRKESSPNSRAKDKFLDQERSNKMRNMAKDKERNQEKPSNSESSLGAKHRLTEEGQEKGKEQERPPEAVSKFAKRNNEETVMSARDRYLARQMARVNAKTYIEKEDD

• Full Name: coiled-coil domain containing 55
• Calculated Molecular Weight: 558 aa, 66 kDa
• Observed Molecular Weight: 66 kDa 70 kDa
• GenBank Accession Number: BC040118
• Gene Symbol: CCDC55
• Gene ID (NCBI): 84081
• RRID: AB_2878847
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• UNIPROT ID: Q9H0G5
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CCDC55, also named as NSRP1 and NSRP70, is a conserved coiled-coil domain containing protein. It is a novel regulator of cell migration. This antibody detects 66 kDa (native form) and 70 kDa (phospho form).

Publications

What published studies show

The CCDC55 (NSRP1) Polyclonal Antibody (Cat# 21360-1-AP) has 1 total citation. It appears in International Journal of Molecular Sciences (2024), in a study on alternative splicing revealing NSRP1's role in promoting adipogenesis through CCDC18. The research field encompasses RNA splicing regulation and adipocyte differentiation. The antibody was applied via Western Blot in pig samples.

Automatically generated from the 1 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
pig	WB	Int J Mol Sci; Comprehensive Atlas of Alternative Splicing Reveals NSRP1 Promoting Adipogenesis through CCDC18; Authors - Lei Liu ; KD Validated