CCDC6 Polyclonal antibody

CCDC6 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 13717-1-AP

Synonyms

Papillary thyroid carcinoma-encoded protein, Coiled-coil domain-containing protein 6, coiled coil domain containing 6

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Validation Data Gallery

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  WB analysis using 13717-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13717-1-AP (CCDC6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IF Staining of MCF-7 using 13717-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CCDC6 antibody (13717-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of MCF-7 using 13717-1-AP

  Immunofluorescent analysis of MCF-7 cells using 13717-1-AP (CCDC6 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HCT 116 cells, HeLa cells, K-562 cells, MCF-7 cells
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 6 publications below

Product Information

13717-1-AP targets CCDC6 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CCDC6 fusion protein Ag4658
• Full Name: coiled-coil domain containing 6
• Calculated Molecular Weight: 474 aa, 53 kDa
• Observed Molecular Weight: 65 kDa
• GenBank Accession Number: BC036757
• Gene Symbol: CCDC6
• Gene ID (NCBI): 8030
• RRID: AB_2291063
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Mol Ther Nucleic Acids; Circular RNA circDNA2 upregulates CCDC6 expression to promote the progression of gastric cancer via miR-149-5p suppression.; Authors - Duochen Jin
human	WB	Mol Cancer Res; Dominant-Negative ATF5 Compromises Cancer Cell Survival by Targeting CEBPB and CEBPD.; Authors - Xiaotian Sun
human	WB	Genes (Basel); Insights into the Evolution of Spermatogenesis-Related Ubiquitin-Proteasome System Genes in Abdominal Testicular Laurasiatherians.; Authors - Xiaoyue Ding
rat	WB	Brain Res Bull; Attenuation of histone H4 lysine 16 acetylation (H4K16ac) elicits a neuroprotection against ischemic stroke by alleviating the autophagic/lysosomal dysfunction in neurons at the penumbra.; Authors - Dong Lingling
human	WB	Anal Bioanal Chem; A gene-encoded FRET fluorescent sensor designed for detecting asymmetric dimethylation levels in vitro and in living cells; Authors - Xuan Sun
human	WB	World J Gastroenterol; Suberoylanilide hydroxamic acid upregulates reticulophagy receptor expression and promotes cell death in hepatocellular carcinoma cells; Authors - Jia-Yao Li