CCDC6 Polyclonal antibody

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• CCDC6 Polyclonal antibody (13717-1-AP)

CCDC6 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 13717-1-AP Copied!

Publications (7)

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Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF/ICC, ELISA

Synonyms

coiled coil domain containing 6, Coiled-coil domain-containing protein 6, Papillary thyroid carcinoma-encoded protein

• 

  WB analysis using 13717-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13717-1-AP (CCDC6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of MCF-7 using 13717-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CCDC6 antibody (13717-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of MCF-7 using 13717-1-AP

  Immunofluorescent analysis of MCF-7 cells using 13717-1-AP (CCDC6 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HCT 116 cells, HeLa cells, K-562 cells, MCF-7 cells
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13717-1-AP targets CCDC6 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag4658

  Product name: Recombinant human CCDC6 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 127-474 aa of BC036757

  Sequence: KETLAVNYEKEEEFLTNELSRKLMQLQHEKAELEQHLEQEQEFQVNKLMKKIKKLENDTISKQLTLEQLRREKIDLENTLEQEQEALVNRLWKRMDKLEAEKRILQEKLDQPVSAPPSPRDISMEIDSPENMMRHIRFLKNEVERLKKQLRAAQLQHSEKMAQYLEEERHMREENLRLQRKLQREMERREALCRQLSESESSLEMDDERYFNEMSAQGLRPRTVSSPIPYTPSPSSSRPISPGLSYASHTVGFTPPTSLTRAGMSYYNSPGLHVQHMGTSHGITRPSPRRSNSPDKFKRPTPPPSPNTQTPVQPPPPPPPPPMQPTVPSAATSQPTPSQHSAHTSSQP

• Full Name: coiled-coil domain containing 6
• Calculated Molecular Weight: 474 aa, 53 kDa
• Observed Molecular Weight: 65 kDa
• GenBank Accession Number: BC036757
• Gene Symbol: CCDC6
• Gene ID (NCBI): 8030
• RRID: AB_2291063
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q16204
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The CCDC6 Polyclonal antibody (Cat# 13717-1-AP) has 7 citations published in World J Gastroenterol, Mol Ther Nucleic Acids, EMBO Rep, Mol Cancer Res, Brain Res Bull, Anal Bioanal Chem, and Genes (Basel). Research fields span hepatocellular carcinoma, gastric cancer progression, cancer cell survival mechanisms (ATF5/CEBPB/CEBPD and FBXO42), neuroprotection against ischemic stroke, FRET-based detection of histone dimethylation, and spermatogenesis-related ubiquitin-proteasome system evolution.

Automatically generated from the 7 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	World J Gastroenterol; Suberoylanilide hydroxamic acid upregulates reticulophagy receptor expression and promotes cell death in hepatocellular carcinoma cells; Authors - Jia-Yao Li
human	WB	Mol Ther Nucleic Acids; Circular RNA circDNA2 upregulates CCDC6 expression to promote the progression of gastric cancer via miR-149-5p suppression.; Authors - Duochen Jin
		EMBO Rep; The regulation of protein phosphatase 4 by FBXO42 is required for cancer cell survival.; Authors - Stephan H Spangenberg
human	WB	Mol Cancer Res; Dominant-Negative ATF5 Compromises Cancer Cell Survival by Targeting CEBPB and CEBPD.; Authors - Xiaotian Sun
rat	WB	Brain Res Bull; Attenuation of histone H4 lysine 16 acetylation (H4K16ac) elicits a neuroprotection against ischemic stroke by alleviating the autophagic/lysosomal dysfunction in neurons at the penumbra.; Authors - Dong Lingling
human	WB	Anal Bioanal Chem; A gene-encoded FRET fluorescent sensor designed for detecting asymmetric dimethylation levels in vitro and in living cells; Authors - Xuan Sun