CCDC62 Polyclonal antibody

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  WB analysis of mouse testis using 25981-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 25981-1-AP (CCDC62 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 25981-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25981-1-AP (CCDC62 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 25981-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25981-1-AP (CCDC62 Antibody) at dilution of 1:50 (under 40x lens).

CCDC62 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 25981-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Synonyms

aaa, CCDC62, Protein aaa, Protein TSP NY, TSP NY

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Tested Applications

• Positive WB detected in: mouse testis tissue
• Positive IHC detected in: human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

25981-1-AP targets CCDC62 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag22043

  Product name: Recombinant human CCDC62 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 265-616 aa of BC060796

  Sequence: DELLNIAKSKQERTNSELHNLRQIYVKQQSDLQFLNFNVENSQELIQMYDSKMEESKALDSSRDMCLSDLENNHPKVDIKREKNQKSLFKDQKFEAMLVQQNRSDKSSCDECKEKKQQIDTVFGEKSVIKLSSIFTKDLVEKHNLPWSLGGKTQIEPENKITLCKIHTKSPKCHGTGVQNEGKQPSETPTLSDEKQWHDVSVYLGLTNCPSSKHPEKLDVECQDQMERSEISCCQKNEACLGESGMCDSKCCHPSNFIIEAPGHMSDVEWMSIFKPSKMQRIVRLKSGCTCSESICGTQHDSPASELIAIQDSHSLGSSKSALREDETESSSNKKNSPTSLLIYKDAPAFNE

• Full Name: coiled-coil domain containing 62
• Calculated Molecular Weight: 684 aa, 78 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC060796
• Gene Symbol: CCDC62
• Gene ID (NCBI): 84660
• RRID: AB_2880318
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q6P9F0
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IF	Elife; scRNA-seq and scATAC-seq reveal that Sertoli cell mediates spermatogenesis disorders through stage-specific communications in non-obstructive azoospermia; Authors - Shimin Wang