CCDC65 Polyclonal antibody

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  WB analysis of mouse testis using 24376-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 24376-1-AP (CCDC65 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat testis using 24376-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 24376-1-AP (CCDC65 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 24376-1-AP

  IP result of anti-CCDC65 (IP:24376-1-AP, 4ug; Detection:24376-1-AP 1:1000) with mouse testis tissue lysate 4000ug.

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  IF Staining of U2OS using 24376-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using CCDC65 antibody (24376-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

CCDC65 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Cat No. 24376-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA

Synonyms

Testis development protein NYD-SP28, NYD SP28, Dynein regulatory complex subunit 2, DRC2, Coiled-coil domain-containing protein 65

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Tested Applications

• Positive WB detected in: mouse testis tissue, rat testis tissue
• Positive IP detected in: mouse testis tissue
• Positive IF/ICC detected in: U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 1 publications below

Product Information

24376-1-AP targets CCDC65 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag19357

  Product name: Recombinant human CCDC65 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 12-325 aa of BC039317

  Sequence: LSDEKQLLLFQQKLLAEEEMAKKKERLLSQFLKDKLAKEEHNSALNLNKINTQWRTVLREVKTRELHKDIEILSQTFERVVDCKDNVIKSLAKDLSEAEEQYAHALRSHLHNVDQLLALQRHRLSLLEESYNMELEALTKEFETERKTIIDQHEKEIHYLQDIFMAMEQNYIDSEYESKLEFQSMWNDLKNMNLEEKHFLRLHLENRVEDLWRKFQDVLKNYTDATEDRKAAFETLQVKDEKSSKEIEVQMKKIQKLQDAITISKGKIMIHSRESEDENRYIRNDKELVLVQLRKLKAQRTQARAASQKNLVRL

• Full Name: coiled-coil domain containing 65
• Calculated Molecular Weight: 484 aa, 57 kDa
• Observed Molecular Weight: 57 kDa
• GenBank Accession Number: BC039317
• Gene Symbol: CCDC65
• Gene ID (NCBI): 85478
• RRID: AB_2879513
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8IXS2
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CCDC65, also known as DRC2, is a sperm tail protein highly expressed in adult testis, spermatocytes, and spermatids. It is a component of the nexin-dynein regulatory complex (N-DRC), a structure important for regulating and orchestrating dynein activity. Mutations in CCDC65 are associated with primary ciliary dyskinesia (PCD), a genetic disorder that affects the structure and function of cilia(PMID: 23991085).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Int J Biol Sci; CCDC65, a Gene Knockout that leads to Early Death of Mice, acts as a potentially Novel Tumor Suppressor in Lung Adenocarcinoma.; Authors - Ziyan Zhang
human	IF	Andrology; A recurrent loss-of-function variant in DRC1 causes non-syndromic severe asthenozoospermia with favorable intracytoplasmic sperm injection and pregnancy outcomes; Authors - Célia Tebbakh