CCDC76 Polyclonal antibody

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  WB analysis using 31484-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31484-1-AP (CCDC76 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 31484-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 31484-1-AP (CCDC76 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 31484-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 31484-1-AP (CCDC76 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 31484-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CCDC76 antibody (31484-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-Phalloidin (green).

CCDC76 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 31484-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

tRNA [Gm4] methyltransferase, TRMT13

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Tested Applications

• Positive WB detected in: HEK-293 cells, K-562 cells, LNCaP cells
• Positive IHC detected in: human placenta tissue, mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31484-1-AP targets CCDC76 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag34110

  Product name: Recombinant human CCDC76 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 132-481 aa of BC075811

  Sequence: GLNSTLKDHIMSHPALHDALNDPKNGDSATKHLKQQASILGNIENLKLLGPRRCFVEFGAGKGKLSHWVDIALKDAEKVHFILVEKVTTRFKVDGKHRKKNSVFERLQIDIQHLCLNKIPVLREEKLPVVGIGKHLCGMATDLALRCLVETYAASFEERNEEPLAKRIKNDKTEKEIYTLAKEGNEKNVPEKWNPVAGIVIALCCHHRCDWRHYVGKEYFRALGLGAVEFHYFQRMSSWATCGMRKTSLETSNSTTKRQDNQNDDSEEHDDGGYRITDDGADCLPGLLSVEEKKKIGHLCKLLIDQGRIQYLQQKGFSPALQYYTDPLVSLENVLLTALPNHSSSPETTA

• Full Name: coiled-coil domain containing 76
• Observed Molecular Weight: 54 kDa
• GenBank Accession Number: BC075811
• Gene Symbol: CCDC76
• Gene ID (NCBI): 54482
• RRID: AB_3670001
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q9NUP7
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CCDC76 (coiled‐coil domain‐containing protein 76), also known as the human tRNA Nm4 methyltransferase (hTrmt13) is an uncharacterized human protein containing 481 amino acid residues that shares 27% sequence similarity with yeast Trm13p. The expression of CCDC76 was significantly associated with patient survival in breast carcinoma and liver and renal cancer patients (PMID: 34850409).