CCDC77 Polyclonal antibody

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• CCDC77 Polyclonal antibody (26369-1-AP)

CCDC77 Polyclonal Antibody for WB, ELISA

Cat No. 26369-1-AP Copied!

Publications (3)

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, ELISA

Synonyms

Coiled-coil domain-containing protein 77

• 

  WB analysis of MCF-7 using 26369-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 26369-1-AP (CCDC77 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 26369-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26369-1-AP (CCDC77 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: MCF-7 cells, A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

26369-1-AP targets CCDC77 in WB, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag14691

  Product name: Recombinant human CCDC77 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 137-488 aa of BC006444

  Sequence: IRELEDKKKIQNLLALVGTDAGEVTYFCKEPPHKVTILQKTIQAVGECEQSESSAFKADPKISKRRPSRERKESSEHYQRDIQTLILQVEALQAQLGEQTKLSREQIEGLIEDRRIHLEEIQVQHQRNQNKIKELTKNLHHTQELLYESTKDFLQLRSENQNKEKSWMLEKDNLMSKIKQYRVQCKKKEDKIGKVLPVMHESHHAQSEYIKSLKDKLVQEKKLSNMYQEQCISLEEELARIREEEGMRREIFKDRTNKMGKRLQIMTKRYEALERRRILEVEGFKTDIKVLRQKLKDLEQMLYKATVNARANQDLALLCEVRDSNRRAHKIQGELKNLKSKVFGLENELRLC

• Full Name: coiled-coil domain containing 77
• Calculated Molecular Weight: 488 aa, 57 kDa
• Observed Molecular Weight: 54-57 kDa
• GenBank Accession Number: BC006444
• Gene Symbol: CCDC77
• Gene ID (NCBI): 84318
• RRID: AB_2880495
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9BR77
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CCDC77 (Coiled-Coil Domain-Containing Protein 77) functions as a critical structural component localized within the centrosome, specifically serving as a core element of the centriolar A-C linker. Depletion of CCDC77 disrupts microtubule triplet cohesion, causing structural destabilization and structural breakage at the proximal end of the centriole (PMID: 40707486).

Publications

What published studies show

The CCDC77 Polyclonal antibody (Cat# 26369-1-AP) has 3 citations. These appear in Cell, Nature Communications, and Autoimmunity. The research fields span centriole molecular architecture and assembly, centriole structural integrity and duplication, and the genetic mechanism of Hashimoto's thyroiditis involving IL-6 signaling.

Automatically generated from the 3 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IF	Cell; Time-series reconstruction of the molecular architecture of human centriole assembly; Authors - Marine H Laporte
human	IF	Nat Commun; The A-C linker controls centriole structural integrity and duplication; Authors - Lorène Bournonville ; KD Validated
human	WB	Autoimmunity; CCDC77 and SLC45A3 mediate the genetic mechanism of Hashimoto's thyroiditis through IL-6.; Authors - Rui Wang ; KD Validated