CCDC78 Polyclonal antibody

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• CCDC78 Polyclonal antibody (26876-1-AP)

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  WB analysis using 26876-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26876-1-AP (CCDC78 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse skeletal muscle using 26876-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 26876-1-AP (CCDC78 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skeletal muscle using 26876-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 26876-1-AP (CCDC78 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CCDC78 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 26876-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Synonyms

C16orf25, Coiled-coil domain-containing protein 78, hsCCDC78, JFP10

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Tested Applications

• Positive WB detected in: C2C12 cells, mouse brain tissue, rat brain tissue
• Positive IHC detected in: mouse skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 3 publications below

Product Information

26876-1-AP targets CCDC78 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag23161

  Product name: Recombinant human CCDC78 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-438 aa of BC042110

  Sequence: MEHAATTGPRPGPPSRRVENVVLRAKDWLPGAPGGTAVWATSLEAEVPPDLALNKEQQLQISKELVDIQITTHHLHEQHEAEIFQLKSEILRLESRVLELELRGDGTSQGCAVPVESDPRHPRAAAQELRHKAQVPGHSDDHRFQVQPKNTMNPENEQHRLGSGLQGEVKWALEHQEARQQALVTRVATLGRQLQGAREEARAAGQRLATQAVVLCSCQGQLRQAEAENARLQLQLKKLKDEYVLRLQHCARQAVEHADGAGQAPATTALRTFLEATLEDIRAAHRSREQQLARAARSYHKRLVDLSRRHEELLVAYRAPGNPQAIFDIASLDLEPLPVPLVTDFSHREDQHGGPGALLSSPKKRPGGASQGGTSEPQGLDAASWAQIHQKLRDFSRSTQSWNGSGHSCWSGPRWLKSNFLSYRSTWTSTWAGTSTKS

• Full Name: coiled-coil domain containing 78
• Observed Molecular Weight: 35-37 kDa
• GenBank Accession Number: BC042110
• Gene Symbol: CCDC78
• Gene ID (NCBI): 124093
• RRID: AB_3669572
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: A2IDD5
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse, human	WB,IF	Nat Commun; A protein complex in the extreme distal tip of vertebrate motile cilia controls their organization, length, and function.; Authors - Juyeon Hong
mouse,human	WB,IF	bioRxiv; Molecular organization of the distal tip of vertebrate motile cilia; Authors - Juyeon Hong
human	WB,IHC,IF	Sci Rep; Integrated bioinformatics and experimental validation identify CCDC78 as a prognostic biomarker in colon adenocarcinoma.; Authors - Qiong Mo ; KD Validated