CCDC8 Polyclonal antibody

CCDC8 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 27194-1-AP

Synonyms

CCDC8

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Validation Data Gallery

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  WB analysis of HEK-293 using 27194-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 27194-1-AP (CCDC8 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 27194-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 27194-1-AP (CCDC8 antibody) at dilution of 1:300.

• 

  IHC staining of human lung cancer using 27194-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27194-1-AP (CCDC8 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 27194-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27194-1-AP (CCDC8 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HEK-293 cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• IF: See 1 publications below

Product Information

The immunogen of 27194-1-AP is CCDC8 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CCDC8 fusion protein Ag25794
• Full Name: coiled-coil domain containing 8
• Observed Molecular Weight: 90 kDa, 59 kDa
• GenBank Accession Number: BC025243
• Gene Symbol: CCDC8
• Gene ID (NCBI): 83987
• RRID: AB_2880795
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Coiled-coil domain-containing protein 8 (CCDC8) is a 538-amino acid protein with the molecular mass of 59kD and 90kD cause of the posttranslational modifications including amidation, glycosylation, phosphorylation, and myristalation (PMID: 21737058). CCDC8 has 2 coiled-coil domains, and the proteins containing this domain are involved in diverse biological processes, such as the regulation of gene expression, cell division, and membrane fusion. CCDC8 is evolutionarily conserved, and CCDC8 mutation leads to the development of 3M syndrome in humans, a primordial growth disorder, by interacting with CUL7, OBSL1, and P53. Low-level or no expression of CCDC8 was shown to be closely related to the development of some tumors, such as renal cell carcinoma, multiple myeloma, breast cancer, and prostate cancer (PMID: 21737058, 27342910).

Publications

Species	Application	Title
human,mouse,rat	IF	Life Sci; Ablation of CCDC8 provides cardioprotection against cardiomyocyte apoptosis via TNF signaling pathway in myocardial ischemia reperfusion injury; Authors - Jungang Huang ; KD Validated

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