CCDC85B Polyclonal antibody

CCDC85B Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 18282-1-AP

Synonyms

Hepatitis delta antigen-interacting protein A, DIPA, Delta-interacting protein A, Delta interacting protein A, Coiled-coil domain-containing protein 85B

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Validation Data Gallery

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  WB analysis of mouse heart using 18282-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 18282-1-AP (CCDC85B Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 18282-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 18282-1-AP (CCDC85B Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 18282-1-AP

  IP result of anti-CCDC85B (IP:18282-1-AP, 4ug; Detection:18282-1-AP 1:300) with mouse heart tissue lysate 3600ug.

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  IHC staining of human liver using 18282-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18282-1-AP (CCDC85B antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 18282-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18282-1-AP (CCDC85B antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HeLa using 18282-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 18282-1-AP (CCDC85B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse heart tissue, mouse lung tissue
• Positive IP detected in: mouse heart tissue
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 1 publications below

Product Information

18282-1-AP targets CCDC85B in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CCDC85B fusion protein Ag13129
• Full Name: coiled-coil domain containing 85B
• Calculated Molecular Weight: 22 kDa
• Observed Molecular Weight: 24-28 kDa
• GenBank Accession Number: BC008796
• Gene Symbol: CCDC85B
• Gene ID (NCBI): 11007
• RRID: AB_2878527
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Coiled-coil domain-containing protein 85B (CCDC85B), also named as DIPA, is 202 amino acid protein, which belongs to the CCDC85 family. CCDC85B localizes in the nucleus and cytoplasm. CCDC85B is widely expressed in many tissues and functions as a transcriptional repressor. CCDC85B may inhibit the activity of CTNNB1 in a TP53-dependent manner and thus regulate cell growth. CCDC85B may play a role in adipocyte differentiation.

Publications

Species	Application	Title
human	IF	Cell Prolif; An artificial intelligence network-guided signature for predicting outcome and immunotherapy response in lung adenocarcinoma patients based on 26 machine learning algorithms; Authors - Nan Zhang
human	WB	Cell; Orphan quality control shapes network dynamics and gene expression; Authors - Kevin G Mark